Tian Yudong, Guan Yanbin, Su Yang, Yang Tao, Yu Haizhou
Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450000, People's Republic of China.
School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan, 450046, People's Republic of China.
Onco Targets Ther. 2021 Feb 23;14:1219-1237. doi: 10.2147/OTT.S282151. eCollection 2021.
Bladder cancer (BLCA) refers to the malignancy growth that spreads from the bladder linings to the bladder muscles. However, the impact of miR-22-3p and lncRNA TRPM2-AS on this tumor has generated divergent views in the literature. This research aimed to study the effects of lncRNA TRPM2-AS on BLCA and its interaction with miR-22-3p and GINS2 mRNA.
qRT-PCR was employed to measure the expression of TRPM2-AS, miR-22-3p and GINS2 mRNA in bladder tissues and cells. The subcellular localization of TRPM2-AS in T24 and 5637 cell lines was identified using a cell fractionation system. Luciferase assay, RIP assay and RNA pull-down assay were later performed to validate the direct binding relationship between TRPM2-AS, miR-22-3p and GINS2 mRNA. Several experiments were conducted to determine the viability, proliferation, colony formation and apoptosis of the cell lines.
Findings indicated that TRPM2-AS was significantly upregulated in BLCA tissues and cell lines. Apart from that, it was observed that TRPM2-AS knockdown significantly inhibited the viability, proliferation and colony formation of BCLA cells, but it promoted the apoptosis of the BCLA cells. A significant downstream target of TRPM2-AS, miR-22-3p was found to show a lower expression level in BLCA tissues and cell lines. However, the inhibition of miR-22-3p considerably enhanced BLCA cell phenotypes. As well as discovering that GINS2 mRNA was a downstream target of miR-22-3p and was significantly upregulated in BLCA, experimental results also indicated that the knockdown of GINS2 suppressed BLCA cell phenotypes.
This research confirmed that TRPM2-AS could promote BCLA by binding to miR-22-3p to increase GINS2 expression. This novel interactome in BLCA cell lines might provide more insights into BLCA therapy.
膀胱癌（BLCA）是指从膀胱内衬扩散到膀胱肌肉的恶性肿瘤生长。然而，miR-22-3p和lncRNA TRPM2-AS对这种肿瘤的影响在文献中产生了不同的观点。本研究旨在探讨lncRNA TRPM2-AS对BLCA的影响及其与miR-22-3p和GINS2 mRNA的相互作用。
采用qRT-PCR检测膀胱组织和细胞中TRPM2-AS、miR-22-3p和GINS2 mRNA的表达。使用细胞分级分离系统鉴定TRPM2-AS在T24和5637细胞系中的亚细胞定位。随后进行荧光素酶报告基因检测、RNA免疫沉淀检测和RNA下拉检测，以验证TRPM2-AS、miR-22-3p和GINS2 mRNA之间的直接结合关系。进行了多项实验以确定细胞系的活力、增殖、集落形成和凋亡情况。
研究结果表明，TRPM2-AS在BLCA组织和细胞系中显著上调。除此之外，观察到敲低TRPM2-AS显著抑制了BCLA细胞的活力、增殖和集落形成，但促进了BCLA细胞的凋亡。发现TRPM2-AS的一个重要下游靶点miR-22-3p在BLCA组织和细胞系中表达水平较低。然而，抑制miR-22-3p可显著增强BLCA细胞表型。同时发现GINS2 mRNA是miR-从膀胱内衬扩散到膀胱肌肉的恶性肿瘤生长。然而，miR-22-3p和lncRNA TRPM2-AS对这种肿瘤的影响在文献中产生了不同的观点。本研究旨在探讨lncRNA TRPM2-AS对BLCA的影响及其与miR-22-3p和GINS2 mRNA的相互作用。
采用qRT-PCR检测膀胱组织和细胞中TRPM2-AS、miR-22-3p和GINS2 mRNA的表达。使用细胞分级分离系统鉴定TRPM2-AS在T24和563
