使用STC-1细胞的胰高血糖素样肽-1释放测定
GLP-1 Release Assay Using STC-1 Cells.
作者信息
Qi Liu, Shuai Tan, Da Jia, Ezra Burstein, Luis Sifuentes-Dominguez
机构信息
Department of Internal Medicine, Division of Digestive and Liver Diseases, University of Texas Southwestern Medical Center, Dallas, United States.
Key Laboratory of Birth Defects and Related Diseases of Women and Children, Department of Pediatrics West China Second University Hospital, State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy, Sichuan University, Chengdu, China.
出版信息
Bio Protoc. 2020 Aug 20;10(16):e3717. doi: 10.21769/BioProtoc.3717.
Abstract
Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC's under fatty acid stimulation.
摘要
肠内分泌细胞（EECs）是胃肠道（GI）上皮中已知的化学传感器。它们会响应各种刺激释放多种肠道激素。在此，我们报告一种体外测定法，用于测量脂肪酸刺激下培养的小鼠EECs释放胰高血糖素样肽-1（GLP-1）的情况。
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