Kini Kossi, Agnimonhan Raoul, Wonni Issa, Silue Drissa
Plant Pathology Unit, AfricaRice, Cotonou, Benin.
Plant Pathology, Institut de l'Environnement et de Recherches Agricoles (INERA), Bobo Dioulasso, Burkina Faso.
Bio Protoc. 2020 Sep 5;10(17):e3740. doi: 10.21769/BioProtoc.3740.
Bacteria blight diseases of rice due to several genera of pathogenic bacteria are one of the major constraints worldwide for rice production. The disease can be best managed through host plant resistance sources. For most of these bacteria such as pv. pv. and subsp. , specific diagnostic techniques that include molecular and pathogenicity tests have been developed. However, for spp., information on pathogenicity assay is very limited and protocols used are not uniform. Most authors use the leaf clipping method. In this paper, we describe the protocol for mechanical inoculation of rice seedlings aged 35 days. The method consists of infiltrating bacterial suspensions at concentrations of 10 CFU/ml, with a needleless syringe into the intercellular and interveinal spaces of rice leaves underside at about 4-5 cm below the leaf tip. This method can be used for a standardized pathogenicity assessment, germplasm resistance evaluation for identifying and characterizing resistance sources.
由几种致病细菌属引起的水稻细菌性条斑病是全球水稻生产的主要限制因素之一。通过寄主植物抗性资源可以最好地控制这种病害。对于大多数这些细菌，如水稻细菌性条斑病菌、水稻白叶枯病菌和稻黄单胞菌稻生致病变种，已经开发了包括分子和致病性测试在内的特定诊断技术。然而，对于黄单胞菌属的细菌，致病性测定的信息非常有限，所使用的方案也不统一。大多数作者使用剪叶法。在本文中，我们描述了对35天大的水稻幼苗进行机械接种的方案。该方法包括用无针注射器将浓度为10CFU/ml的细菌悬浮液注入叶尖下方约4-5厘米处水稻叶片下侧的细胞间隙和叶脉间空间。该方法可用于标准化的致病性评估、种质抗性评价，以鉴定和表征抗性资源。
