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利用多重聚合酶链反应同时检测水稻中的三种细菌性种传病害

Simultaneous Detection of Three Bacterial Seed-Borne Diseases in Rice Using Multiplex Polymerase Chain Reaction.

作者信息

Kang In Jeong, Kang Mi-Hyung, Noh Tae-Hwan, Shim Hyeong Kwon, Shin Dong Bum, Heu Suggi

机构信息

Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration, Suwon 16429, Korea.

Capacity Building Division, Rural Development Administration, Jeonju 54875, Korea.

出版信息

Plant Pathol J. 2016 Dec;32(6):575-579. doi: 10.5423/PPJ.NT.05.2016.0118. Epub 2016 Dec 1.

DOI:10.5423/PPJ.NT.05.2016.0118
PMID:27904465
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5117867/
Abstract

(bacterial grain rot), pv. (bacterial leaf blight), and subsp. (bacterial brown stripe) are major seedborne pathogens of rice. Based on the 16S and 23S rDNA sequences for subsp. and , and transposase A gene sequence for pv. , three sets of primers had been designed to produce 402 bp for , 490 bp for , and 290 bp for subsp. with the 63°C as an optimum annealing temperature. Samples collected from naturally infected fields were detected with two bacteria, and subsp. but pv. was not detected. This assay can be used to identify pathogens directly from infected seeds, and will be an effective tool for the identification of the three pathogens in rice plants.

摘要

（细菌性谷粒腐烂病）、稻黄单胞菌稻致病变种（细菌性叶枯病）和稻黄单胞菌稻生致病变种（细菌性褐条病）是水稻主要的种传病原菌。基于稻黄单胞菌稻生致病变种和稻黄单胞菌稻致病变种的16S和23S rDNA序列，以及稻黄单胞菌稻致病变种的转座酶A基因序列，设计了三组引物，分别用于扩增稻黄单胞菌稻生致病变种的402 bp片段、稻黄单胞菌稻致病变种的490 bp片段和稻黄单胞菌稻生致病变种的290 bp片段，最佳退火温度为63°C。从自然感染田采集的样本检测出了两种细菌，即稻黄单胞菌稻生致病变种和稻黄单胞菌稻生致病变种，但未检测到稻黄单胞菌稻致病变种。该检测方法可直接从感染种子中鉴定病原菌，将成为鉴定水稻植株中这三种病原菌的有效工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c9e/5117867/482253a27f20/ppj-32-575f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c9e/5117867/54a71949ecef/ppj-32-575f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c9e/5117867/45216e460cd6/ppj-32-575f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c9e/5117867/53992366473f/ppj-32-575f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c9e/5117867/36d280b12d28/ppj-32-575f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c9e/5117867/482253a27f20/ppj-32-575f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c9e/5117867/54a71949ecef/ppj-32-575f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c9e/5117867/45216e460cd6/ppj-32-575f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c9e/5117867/53992366473f/ppj-32-575f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c9e/5117867/36d280b12d28/ppj-32-575f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c9e/5117867/482253a27f20/ppj-32-575f5.jpg

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本文引用的文献

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Plant Dis. 2003 Aug;87(8):890-895. doi: 10.1094/PDIS.2003.87.8.890.
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Burkholderia glumae: next major pathogen of rice?引起谷子细菌性斑点病的伯氏菌：水稻的下一个主要病原菌？
Mol Plant Pathol. 2011 May;12(4):329-39. doi: 10.1111/j.1364-3703.2010.00676.x. Epub 2010 Nov 24.
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The epidemiology and management of seedborne bacterial diseases.
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Class III Histidine Kinases: a Recently Accessorized Kinase Domain in Putative Modulators of Type IV Pilus-Based Motility.III类组氨酸激酶：IV型菌毛运动性推定调节因子中最近获得的激酶结构域。
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