邻近连接杂交技术（PLISH）的进展。
Advances in Proximity Ligation Hybridization (PLISH).
作者信息
Nagendran Monica, Andruska Adam M, Harbury Pehr B, Desai Tushar J
机构信息
Department of Internal Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, Stanford University School of Medicine, Stanford, California, USA.
Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA.
出版信息
Bio Protoc. 2020 Nov 5;10(21):e3808. doi: 10.21769/BioProtoc.3808.
Understanding tissues in the context of development, maintenance and disease requires determining the molecular profiles of individual cells within their native spatial context. We developed a Proximity Ligation Hybridization technology (PLISH) that enables quantitative measurement of single cell gene expression in intact tissues, which we have now updated. By recording spatial information for every profiled cell, PLISH enables retrospective mapping of distinct cell classes and inference of their interactions. PLISH has high sensitivity, specificity and signal to noise ratio. It is also rapid, scalable, and does not require expertise in molecular biology so it can be easily adopted by basic and clinical researchers.
要在发育、维持和疾病的背景下理解组织，需要在其原生空间背景中确定单个细胞的分子谱。我们开发了一种邻近连接杂交技术（PLISH），它能够对完整组织中的单细胞基因表达进行定量测量，我们现在已经对其进行了更新。通过记录每个被分析细胞的空间信息，PLISH能够对不同细胞类别进行回顾性映射，并推断它们之间的相互作用。PLISH具有高灵敏度、特异性和信噪比。它还快速、可扩展，并且不需要分子生物学方面的专业知识，因此基础研究人员和临床研究人员都可以轻松采用。
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