Kohyama Shunshi, Fujiwara Kei, Yoshinaga Natsuhiko, Doi Nobuhide
Department of Biosciences and Informatics, Keio University, Yokohama, Japan.
Mathematical Science Group, WPI Advanced Institute for Materials Research (WPI-AIMR), Tohoku University, Sendai, Japan.
Bio Protoc. 2020 Mar 20;10(6):e3561. doi: 10.21769/BioProtoc.3561.
The Min system determines the cell division plane of bacteria. As a cue of spatiotemporal regulation, the Min system uses wave propagation of MinD protein (Min wave). Therefore, the reconstitution of the Min wave in cell-sized closed space will lead to the creation of artificial cells capable of cell division. The Min waves emerge via coupling between the reactions among MinD, MinE, and ATP and the differences in diffusion rate on the cell membrane and in the cytoplasm. Because Min waves appear only under the balanced condition of the reaction-diffusion coupling, special attentions are needed towards several technical points for the reconstitution of Min waves in artificial cells. This protocol describes a technical method for stably generating Min waves in artificial cells.
Min系统决定细菌的细胞分裂平面。作为一种时空调节线索，Min系统利用MinD蛋白的波传播（Min波）。因此，在细胞大小的封闭空间中重建Min波将导致能够进行细胞分裂的人工细胞的产生。Min波通过MinD、MinE和ATP之间的反应以及细胞膜和细胞质中扩散速率的差异之间的耦合而出现。由于Min波仅在反应-扩散耦合的平衡条件下出现，因此在人工细胞中重建Min波时需要特别注意几个技术要点。本方案描述了一种在人工细胞中稳定产生Min波的技术方法。
