Biram Adi, Shulman Ziv
Department of Immunology, Weizmann Institute of Science, Rehovot, Israel.
Bio Protoc. 2020 May 5;10(9):e3602. doi: 10.21769/BioProtoc.3602.
Generation of antibodies is crucial for establishing enduring protection from invading pathogens, as well as for maintaining homeostasis with commensal bacteria at mucosal surfaces. Chronic exposure to microbiota- and dietary- derived antigens results in continuous production of antibody producing cells within the Peyer's patch germinal center structures. Recently, we have shown that B cells responding to gut-derived antigens colonize the subepithelial dome (SED) in Peyer's patches and rapidly proliferate independently of their relative BCR affinity. To evaluate B cell proliferation within different niches in Peyer's patches, we applied EdU incorporation assay as described in this protocol.
产生抗体对于建立抵御入侵病原体的持久保护以及维持黏膜表面与共生细菌的内环境稳定至关重要。长期暴露于微生物群和饮食来源的抗原会导致派尔集合淋巴结生发中心结构内持续产生抗体产生细胞。最近，我们发现对肠道来源抗原作出反应的B细胞定位于派尔集合淋巴结的上皮下圆顶区（SED），并独立于其相对BCR亲和力快速增殖。为了评估派尔集合淋巴结不同微环境中的B细胞增殖情况，我们按照本方案所述应用了EdU掺入试验。
