Zhang Gaixia, Wang Hui, Jiang Jiemei, Wang Qiuling, Li Baoli, Shi Linchun, Wei Jianhe
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Institute of Sericulture, Chengde Medical University, Chengde, China.
Mitochondrial DNA B Resour. 2021 Feb 9;6(2):441-443. doi: 10.1080/23802359.2020.1870899.
The root of var. (H. Wolff) Shan & Yin Li (Apiaceae), a new substitution for the popular Chinese medicinal material, Bupleuri Radix (Chai hu), is not easily distinguishable via traditional methods. The complete chloroplast genome sequence of var. was characterized using next-generation sequencing and the assembly method. The complete genome was 155,576 bp in length and contained two inverted repeat (IR) regions of 26,311 bp, a large single-copy (LSC) region of 85,351 bp, and a small single-copy (SSC) region of 17,603 bp. It encoded 113 unique genes consisting of 79 protein-coding genes (PCGs), 30 transfer RNA genes, and four ribosomal RNA genes. Importantly, three genes (, and ) with small exon, and one trans-splicing gene () were correctly annotated. The overall GC content of the var. chloroplast genome is 37.7%. The phylogenetic analyses indicated that var. was closely related to . Moreover, many genetic information sites were available for distinguishing var. from the official 'Chai hu' plant sources, Willd. and DC.
柴胡变种（H. Wolff） Shan & Yin Li（伞形科）的根是常用中药材柴胡的新替代品，通过传统方法不易区分。利用二代测序和组装方法对柴胡变种的叶绿体全基因组序列进行了特征分析。该全基因组长度为155,576 bp，包含两个26,311 bp的反向重复（IR）区域、一个85,351 bp的大单拷贝（LSC）区域和一个17,603 bp的小单拷贝（SSC）区域。它编码113个独特基因，包括79个蛋白质编码基因（PCG）、30个转运RNA基因和4个核糖体RNA基因。重要的是，正确注释了3个外显子较小的基因（、和）以及1个反式剪接基因（）。柴胡变种叶绿体基因组的总体GC含量为37.7%。系统发育分析表明，柴胡变种与密切相关。此外，有许多遗传信息位点可用于区分柴胡变种与法定的“柴胡”植物来源，即狭叶柴胡Willd.和柴胡DC。
