Zhong Yao Cai. 2016 Sep;39(9):1975-81.
Macroscopic identification, thin layer chromatography( TLC) identification and molecular identification based on the Chinese Pharmacopoeia were established to identify five kinds of Bupleurum medicinal materials such as Bupleurum chinense,Bupleurum scorzonerifolium, Bupleurum marginatum, Bupleurum marginatum var. stenophyllum and Bupleurum bicaule,which were easily confused.
According to the Chinese Pharmacopoeia,macroscopic characters of these five kinds of Bupleurum medicinal materials were observed and described; TLC identification method with saikosaponin d and control medicinal material of Bupleuri Radix as reference substances was established. Furthermore, DNA barcode was applied in identification of these confused drugs.
Five Bupleurum medicinal materials could be easily distinguished by their morphological characters. Moreover, TLC of Bupleurum chinense,Bupleurum scorzonerifolium, Bupleurum marginatum and Bupleurum marginatum var. stenophyllum were extremely similar while being different from that of Bupleurum bicaule. The ITS2 sequences could be used to identify the five Bupleurum medicinal materials.
The established macroscopic identification and TLC for distinguishing of the five kinds of Bupleurum medicinal materials are simple and accurate while DNA barcode technology being effective and extensive. Besides, this study provides scientific basis for the further pharmacodynamic studies on Bupleurum marginatum and Bupleurum marginatum var. stenophyllum.
基于《中国药典》建立宏观鉴定、薄层色谱（TLC）鉴定及分子鉴定方法，以鉴别易混淆的5种柴胡属药用植物，即柴胡、狭叶柴胡、膜缘柴胡、狭叶膜缘柴胡和双茎柴胡。
依据《中国药典》，观察并描述这5种柴胡属药用植物的宏观特征；建立以柴胡皂苷d为对照品、柴胡对照药材为对照的TLC鉴别方法。此外，应用DNA条形码技术对这些易混淆药材进行鉴定。
5种柴胡属药用植物可通过形态特征轻易区分。此外，柴胡、狭叶柴胡、膜缘柴胡和狭叶膜缘柴胡的TLC图谱极为相似，而与双茎柴胡的不同。ITS2序列可用于鉴别这5种柴胡属药用植物。
所建立的用于鉴别5种柴胡属药用植物的宏观鉴定和TLC方法简便准确，而DNA条形码技术有效且应用广泛。此外，本研究为膜缘柴胡和狭叶膜缘柴胡进一步的药效学研究提供了科学依据。
