Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education and National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou 570311, China.
Molecules. 2024 May 29;29(11):2561. doi: 10.3390/molecules29112561.
Volatile oil serves as a traditional antipyretic component of Bupleuri Radix. var. (Wolff) Shan et Y. Li belongs to the genus and is distinguished for its high level of saikosaponins and volatile oils; nonetheless, prevailing evidence remains inconclusive regarding its viability as an alternative resource of other official species. This study aims to systematically compare the volatile oil components of both dried and fresh roots of var. and the four legally available species across their chemical, molecular, bionics, and anatomical structures. A total of 962 compounds were determined via GC-MS from the dried roots; var. showed the greatest differences from other species in terms of hydrocarbons, esters, and ketones, which was consistent with the results of fresh roots and the e-nose analysis. A large number of DEGs were identified from the key enzyme family of the monoterpene synthesis pathway in var. via transcriptome analysis. The microscopic observation results, using different staining methods, further showed the distinctive high proportion of phloem in var. , the structure which produces volatile oils. Together, these pieces of evidence hold substantial significance in guiding the judicious development and utilization of genus resources.
挥发油是柴胡属（Bupleuri Radix）传统的解热成分。 var. （Wolff）Shan et Y. Li 因其高水平的柴胡皂苷和挥发油而被区分开来；然而，关于其作为其他官方物种替代资源的可行性，仍存在不确定的证据。本研究旨在系统比较干燥和新鲜的 var. 根与四种合法的柴胡属物种的挥发油成分，包括它们的化学、分子、仿生学和解剖结构。通过 GC-MS 从干燥的根中共鉴定出 962 种化合物； var. 与其他物种在烃类、酯类和酮类方面差异最大，这与新鲜根和电子鼻分析的结果一致。通过转录组分析，从单萜合成途径的关键酶家族中鉴定出大量差异表达基因（DEGs）。使用不同染色方法的微观观察结果进一步显示，var. 的韧皮部比例很高，这是产生挥发油的结构。这些证据具有重要意义，可指导柴胡属资源的合理开发和利用。
