非模式物种中转座酶可及染色质测序分析的协议。

School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK.

STAR Protoc. 2021 Feb 13;2(1):100341. doi: 10.1016/j.xpro.2021.100341. eCollection 2021 Mar 19.

The assay for transposase accessible chromatin (ATAC-seq) is a method for mapping genome-wide chromatin accessibility. Coupled with high-throughput sequencing, it enables integrative epigenomics analyses. ATAC-seq requires direct access to cell nuclei, a major challenge in non-model species such as small invertebrates, whose soft tissue is surrounded by a protective exoskeleton. Here, we present modifications of the ATAC-seq protocol for applications in small crustaceans, extending applications to non-model species. For complete information on the use and execution of this protocol, please refer to Buenrostro et al. (2013).

转座酶可及染色质分析（ATAC-seq）是一种用于绘制全基因组染色质可及性图谱的方法。与高通量测序相结合，它能够进行综合表观基因组学分析。ATAC-seq 需要直接接触细胞核，这对于小型无脊椎动物等非模式物种来说是一个主要挑战，因为它们的软组织被保护性外骨骼所包围。在这里，我们对 ATAC-seq 方案进行了修改，以应用于小型甲壳类动物，将其应用扩展到非模式物种。有关该方案使用和执行的完整信息，请参考 Buenrostro 等人（2013 年）。