National Key Laboratory of Plant Molecular Genetics (NKLPMG), CAS center for Excellence in Molecular Plant Science, Institute of Plant Physiology and Ecology (SIPPE), Chinese Academy of Sciences (CAS), Shanghai 200032, China.
University of Chinese Academy of Sciences (UCAS), Shanghai 200032, China.
STAR Protoc. 2021 Jan 21;2(1):100289. doi: 10.1016/j.xpro.2020.100289. eCollection 2021 Mar 19.
Open or accessible regions of the genome are the primary positions of binding sites for transcription factors and chromatin regulators. Transposase-accessible chromatin sequencing (ATAC-seq) can probe chromatin accessibility in the intact nucleus. Here, we describe a protocol to generate ATAC-seq libraries from fresh tissues and establish an easy-to-use bioinformatic analysis pipeline. Our method could be applied to other plants and other tissues and allows for the reliable detection of changes in chromatin accessibility throughout plant growth and development. For complete details on the use and execution of this protocol, please refer to Wang et al. (2020).
基因组的开放或可及区域是转录因子和染色质调控因子结合位点的主要位置。转座酶可及染色质测序（ATAC-seq）可探测完整核内染色质的可及性。本文我们描述了一种从新鲜组织中生成 ATAC-seq 文库的方案，并建立了一个易于使用的生物信息学分析流程。我们的方法可应用于其他植物和其他组织，并可可靠检测到植物生长和发育过程中染色质可及性的变化。有关该方案使用和执行的完整详细信息，请参见 Wang 等人（2020 年）。
