通过胸苷酸饥饿筛选酵母营养缺陷型。
Selection of yeast auxotrophs by thymidylate starvation.
作者信息
Barclay B J, Little J G
出版信息
J Bacteriol. 1977 Dec;132(3):1036-7. doi: 10.1128/jb.132.3.1036-1037.1977.
Abstract
A rapid procedure for the recovery of Saccharomyces cerevisiae auxotrophs was developed by exploiting the protection of these mutants from thymineless death when a required metabolite was withheld. The method can be used for thymidine 5'-monophosphate-requiring auxotrophs or wild-type strains blocked in de novo synthesis of thymidylate by folate antagonists.
摘要
通过利用当所需代谢物被扣留时这些突变体免受无胸腺嘧啶死亡的保护，开发了一种快速回收酿酒酵母营养缺陷型的方法。该方法可用于需要5'-单磷酸胸苷的营养缺陷型或因叶酸拮抗剂而在胸苷酸从头合成中受阻的野生型菌株。
相似文献
1
Selection of yeast auxotrophs by thymidylate starvation.通过胸苷酸饥饿筛选酵母营养缺陷型。
J Bacteriol. 1977 Dec;132(3):1036-7. doi: 10.1128/jb.132.3.1036-1037.1977.
2
Thymidine 5'-monophosphate-requiring mutants of Saccharomyces cerevisiae are deficient in thymidylate synthetase.酿酒酵母中需要胸苷5'-单磷酸的突变体缺乏胸苷酸合成酶。
J Bacteriol. 1977 Oct;132(1):44-50. doi: 10.1128/jb.132.1.44-50.1977.
3
Isolation and characterization of yeast mutants auxotrophic for 2'-deoxythymidine 5'-monophosphate.对2'-脱氧胸苷5'-单磷酸营养缺陷型酵母突变体的分离与鉴定。
Mol Gen Genet. 1979 Jan 10;168(2):141-51. doi: 10.1007/BF00431440.
4
Effects of excess thymidylate on thymidylate low-requiring strains of Saccharomyces cerevisiae: high mutagenicity and absence of DNA strand breaks.过量胸苷酸对酿酒酵母胸苷酸低需求菌株的影响：高诱变性及无DNA链断裂
Mutat Res. 1988 Jan;197(1):59-66. doi: 10.1016/0027-5107(88)90140-6.
5
Genetic and biochemical consequences of thymidylate stress.胸苷酸应激的遗传和生化后果。
Can J Biochem. 1982 Mar;60(3):172-84. doi: 10.1139/o82-023.
6
Nucleic acid metabolism in yeast II. Metabolism of thymidylate during thymidylate excess death.酵母中的核酸代谢II.胸苷酸过量致死期间胸苷酸的代谢
Mol Gen Genet. 1981;182(1):60-4. doi: 10.1007/BF00422767.
7
Enhanced canavanine uptake is associated with nucleotide permeability in a thymidylate auxotroph of Saccharomyces cerevisiae.在酿酒酵母的胸苷酸营养缺陷型中，刀豆氨酸摄取增强与核苷酸通透性相关。
Curr Genet. 1989 Feb;15(2):129-34. doi: 10.1007/BF00435459.
8
A simple method for the isolation and characterization of thymidylate uptaking mutants in Saccharomyces cerevisiae.一种用于分离和鉴定酿酒酵母胸苷酸摄取突变体的简单方法。
Mol Gen Genet. 1976 Aug 19;147(2):209-15. doi: 10.1007/BF00267573.
9
Isolation and properties of yeast mutants with highly efficient thymidylate utilization.高效利用胸苷酸的酵母突变体的分离与特性
Z Naturforsch C Biosci. 1976 Jul-Aug;31(7-8):468-78. doi: 10.1515/znc-1976-7-824.
10
Nucleic acid metabolism in yeast, V. Excretion of thymidylate.酵母中的核酸代谢，V. 胸苷酸的排泄
Mol Gen Genet. 1982;188(1):115-20. doi: 10.1007/BF00333004.
引用本文的文献
1
Thymine-starvation-induced chromosomal fragmentation is not required for thymineless death in Escherichia coli.胸腺嘧啶饥饿诱导的染色体碎片化对于大肠杆菌中的无胸腺嘧啶死亡不是必需的。
Mol Microbiol. 2022 May;117(5):1138-1155. doi: 10.1111/mmi.14897. Epub 2022 Apr 25.
2
Inactivation of folylpolyglutamate synthetase Met7 results in genome instability driven by an increased dUTP/dTTP ratio.甲硫氨酸 7 号残基的叶酸多聚谷氨酸合成酶失活导致 dUTP/dTTP 比值升高引发的基因组不稳定。
Nucleic Acids Res. 2020 Jan 10;48(1):264-277. doi: 10.1093/nar/gkz1006.
3
Cytocidal amino acid starvation of Saccharomyces cerevisiae and Candida albicans acetolactate synthase (ilv2{Delta}) mutants is influenced by the carbon source and rapamycin.酿酒酵母和白假丝酵母乙酰乳酸合酶（ilv2Δ）突变体的细胞毒性氨基酸饥饿受碳源和雷帕霉素的影响。
Microbiology (Reading). 2010 Mar;156(Pt 3):929-939. doi: 10.1099/mic.0.034348-0. Epub 2009 Dec 17.
4
dUTP pyrophosphatase is an essential enzyme in Saccharomyces cerevisiae.脱氧尿苷三磷酸焦磷酸酶是酿酒酵母中的一种必需酶。
EMBO J. 1993 Nov;12(11):4425-31. doi: 10.1002/j.1460-2075.1993.tb06127.x.
5
Enrichment for auxotrophic mutants in Saccharomyces cerevisiae using the cell wall inhibitor, echinocandin B.使用细胞壁抑制剂棘白菌素B筛选酿酒酵母中的营养缺陷型突变体
Mol Gen Genet. 1982;186(2):295-7. doi: 10.1007/BF00331865.
6
Selection and fusion of auxotrophic protoplasts of Candida albicans.白色念珠菌营养缺陷型原生质体的筛选与融合
Antonie Van Leeuwenhoek. 1982 May;48(2):169-82. doi: 10.1007/BF00405201.
7
Parasexual genetic analysis of Candida albicans by spheroplast fusion.通过原生质体融合对白色念珠菌进行准性遗传分析。
J Bacteriol. 1981 Jun;146(3):833-40. doi: 10.1128/jb.146.3.833-840.1981.
8
Induction of mitotic recombination in yeast by starvation for thymine nucleotides.胸腺嘧啶核苷酸饥饿诱导酵母中的有丝分裂重组。
Proc Natl Acad Sci U S A. 1980 Oct;77(10):6057-61. doi: 10.1073/pnas.77.10.6057.
9
Enhanced canavanine uptake is associated with nucleotide permeability in a thymidylate auxotroph of Saccharomyces cerevisiae.在酿酒酵母的胸苷酸营养缺陷型中，刀豆氨酸摄取增强与核苷酸通透性相关。
Curr Genet. 1989 Feb;15(2):129-34. doi: 10.1007/BF00435459.
10
Growth inhibition of Candida albicans by folate pathway inhibitors. Their potential in the selection of auxotrophs.叶酸途径抑制剂对白色念珠菌的生长抑制作用。它们在选择营养缺陷型菌株方面的潜力。
Antonie Van Leeuwenhoek. 1979;45(2):211-23. doi: 10.1007/BF00418585.
本文引用的文献
1
Selection of spontaneous mutants by inositol starvation in yeast.通过酵母肌醇饥饿筛选自发突变体。
Mol Gen Genet. 1975 Dec 30;143(1):5-11. doi: 10.1007/BF00269415.
Zotero 插件