Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416 Gdańsk, Poland.
Food Funct. 2021 Mar 21;12(6):2686-2692. doi: 10.1039/d1fo00226k. Epub 2021 Mar 4.
The on-line heart-cutting two-dimensional liquid chromatography method with the use of a diode array detector and a mass spectrometer (LC-LC-DAD-ESI-MS) was established and validated for quantitation of C-glycosylflavones in fenugreek seeds (Foenugraeci semen, Trigonella foenum-graecum L.). The first- (D) and second- (D) dimensional separations were performed on Kinetex C-18 columns with different diameters, respectively, and gradient (D) and isocratic elution (D). Finally, 17 compounds were separated, 13 of which were quantified by D separation and 4 compounds by D separation. As a result, it was pointed out that fenugreek seeds of Polish origin can be considered as a rich source of C-glycosylflavones. Antibacterial activity against Helicobacter pylori of standardized 70% methanol extract from fenugreek seeds has been demonstrated, in contrast to the inactive aqueous extract. Anti-H. pylori activity of the 70% methanol extract can be related to a higher concentration of C-glycosylflavones. This is the first report on the bactericidal activity of vitexin, diosgenin, tigogenin and sarsasapogenin against H. pylori and the bacteriostatic activity of orientin against this bacterium.
建立并验证了在线心切割二维液相色谱法，该方法使用二极管阵列检测器和质谱仪（LC-LC-DAD-ESI-MS），用于定量测定葫芦巴种子（葫芦巴种子，Trigonella foenum-graecum L.）中的 C-糖苷黄酮。分别在不同直径的 Kinetex C-18 柱上进行第一维和第二维分离，分别进行梯度（D）和等度洗脱（D）。最终，分离出 17 种化合物，其中 13 种通过 D 分离定量，4 种通过 D 分离定量。结果表明，波兰产葫芦巴种子可被视为 C-糖苷黄酮的丰富来源。与无活性的水提物相比，标准化 70%甲醇提取物对幽门螺杆菌具有抗菌活性。70%甲醇提取物的抗 H. pylori 活性可能与 C-糖苷黄酮的浓度更高有关。这是首次报道牡荆素、薯蓣皂苷元、远志皂苷元和知母皂苷元对 H. pylori 的杀菌活性以及对该细菌的Orientin 的抑菌活性。
