Oh Dae-Ju, Lee Jong-Chul, Ham Young-Min, Jung Yong-Hwan
Biodiversity Research Institute, Jeju Technopark, Seogwipo, Republic of Korea.
Genet Mol Biol. 2021 Mar 3;44(1):e20200166. doi: 10.1590/1678-4685-GMB-2020-0166. eCollection 2021.
The complete mitochondrial (mt) genome of Stereolepis doederleini was sequenced from a specimen collected in a commercial aquarium in Jeju Island. The sequence was 16,513 base pairs in length and, similar to other vertebrate mt genomes, included 37 mt genes and a noncoding control region; the gene order was identical to that of typical vertebrate mt genome. Mitochondrial genome sequences of 17 species from 12 families were used to reconstruct phylogenetic relationships within the order Pempheriformes. The phylogenetic trees were constructed with three methods (neighbor joining [NJ], maximum likelihood [ML], and Bayesian method) using 12 protein coding genes, but not ND6. In all phylogenetic trees, Pempheriformes were clustered into three strongly supported clades. Two Acropomatidae species (Synagrops japonicus in clade-Ⅰ and Doederleinia berycoides in clade-Ⅲ) were polyphyletic; S. japonicus was close to Lateolabracidae and was the sister of Glaucosomatidae + (Pempheridae/(Percophidae+Creediidae)), and D. berycoides was sister to Howellidae + Epigonidae. All phylogenetic trees supported a sister relationship between Creediidae and Percophidae in clade-Ⅰ. Glaucosomatidae formed a sister clade with Pempheridae. The relationships within clade-Ⅱ, which was composed of four families (Pentacerotidae, Polyprionidae, Banjosidae, and Bathyclupeidae), slightly differed between NJ/ML and BI tree topologies. In clade-Ⅲ, the relationships among Howellidae, Epigonidae, and Acropomatidae were strongly supported.
从济州岛一家商业水族馆采集的杜氏棘鲷标本中，对其完整的线粒体（mt）基因组进行了测序。该序列长度为16,513个碱基对，与其他脊椎动物的线粒体基因组相似，包含37个线粒体基因和一个非编码控制区；基因顺序与典型脊椎动物线粒体基因组相同。利用12个科17个物种的线粒体基因组序列重建了发光鲷目内部的系统发育关系。使用12个蛋白质编码基因（不包括ND6），通过三种方法（邻接法[NJ]、最大似然法[ML]和贝叶斯法）构建系统发育树。在所有系统发育树中，发光鲷目被聚类为三个得到有力支持的分支。两种发光鲷科物种（分支Ⅰ中的日本光颌鮨和分支Ⅲ中的杜氏棘鲷）是多系的；日本光颌鮨与鲈科关系密切，是银鲈科 + （发光鲷科/（发光鲷科+科氏发光鲷科））的姐妹种，而杜氏棘鲷是豪氏发光鲷科 + 后颌鱼科的姐妹种。所有系统发育树都支持分支Ⅰ中科氏发光鲷科和发光鲷科之间的姐妹关系。银鲈科与发光鲷科形成姐妹分支。由四个科（五棘鲷科、石斑鱼科、斑鲈科和深海鲱科）组成的分支Ⅱ内的关系在NJ/ML和BI树拓扑结构之间略有不同。在分支Ⅲ中，豪氏发光鲷科、后颌鱼科和发光鲷科之间的关系得到有力支持。
