Faculty of Life Sciences and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
Appl Biochem Biotechnol. 2021 Oct;193(10):3173-3186. doi: 10.1007/s12010-021-03593-x. Epub 2021 Jun 5.
To maintain high microalgae lipid productivity and flocculation efficiency simultaneously and reduce the production cost of microalgae lipids, Monoraphidium sp. FXY-10 with high lipid-producing capacity and Heveochlorella sp. Yu with strong self-flocculation ability were cocultivated and studied. Cocultivated microalgae lipid productivity and flocculation efficiency were increased to 203.8 mg L day and 70.55%, respectively, which is potentially related to the excessive competitive depletion of nitrogen sources and the upregulation of correlative key genes in lipid anabolic metabolism. Under cocultivation conditions, microalgae cells could enter the stationary phase 2 days earlier than that under monocultivation conditions, thus reducing the culture time. Relative expression of the accD, ME, and rbcL genes was upregulated to varying degrees, and the enzyme activities of ACCase, ME, and RuBisCO were also significantly increased compared with those in monocultivation. Moreover, fatty acid composition showed that microalgae lipids in cocultivation exhibited potential as a feedstock for biodiesel.
为了同时保持高微藻油脂生产力和絮凝效率，并降低微藻油脂的生产成本，本研究对具有高产油能力的单胞藻属 FXY-10 和具有强自絮凝能力的雨生红球藻属 Yu 进行了共培养和研究。共培养的微藻油脂生产力和絮凝效率分别提高到 203.8 mg L day 和 70.55%，这可能与氮源的过度竞争消耗和脂质合成代谢相关关键基因的上调有关。在共培养条件下，微藻细胞比在单培养条件下提前 2 天进入静止期，从而缩短了培养时间。accD、ME 和 rbcL 基因的相对表达水平均有不同程度的上调，ACCase、ME 和 RuBisCO 的酶活性也明显高于单培养。此外，脂肪酸组成表明，共培养的微藻油脂具有作为生物柴油原料的潜力。
