Tone O, Miller J C, Bell J M, Rapoport S I
Laboratory of Neurosciences, National Institute on Aging, Bethesda, Maryland 20892.
Exp Neurol. 1988 Jun;100(3):491-505. doi: 10.1016/0014-4886(88)90034-9.
Regional cerebral incorporation of intravenously injected [U-14C]palmitate was measured from 1 day to 13 weeks after left cochlear destruction in 11-day- and 3 month-old, awake Fischer-344 rats. In 11-day-old animals, statistically significant left-right differences in incorporation were absent 1 day after cochlear destruction and were found only in parts of the cochlear nucleus and inferior colliculus after 1 week. After 6 to 13 weeks, consistent with functional neuroanatomy of central auditory regions, incorporation was reduced by 6 to 9% in the left cochlear nucleus and left lateral superior olivary nucleus, compared with corresponding right-side regions. The right medial superior olivary nucleus, medial nucleus of the trapezoid body, lateral lemniscus nucleus, inferior colliculus, medial geniculate body, and auditory cortex had 5 to 9% less incorporation than did corresponding left-side regions. Fewer significant differences after chronic auditory deprivation occurred in 3-month-old rats than in 11-day-old rats following cochlear destruction. Reduced incorporation corresponded to reported changes in cell morphology, which also were greater in immature than mature rats following auditory deprivation. The results suggest that the palmitate method can be used to identify long-term regional changes in the turnover of brain lipids after sensory deprivation.
在11日龄和3月龄的清醒Fischer-344大鼠中，于左侧耳蜗破坏后1天至13周测量静脉注射的[U-14C]棕榈酸酯在大脑区域的摄取情况。在11日龄动物中，耳蜗破坏后1天，摄取量不存在统计学上显著的左右差异，仅在1周后于部分耳蜗核和下丘中发现差异。6至13周后，与中枢听觉区域的功能神经解剖学一致，左侧耳蜗核和左侧外侧上橄榄核的摄取量相较于相应右侧区域减少了6%至9%。右侧内侧上橄榄核、斜方体内侧核、外侧丘系核、下丘、内侧膝状体和听觉皮层的摄取量比相应左侧区域少5%至9%。与11日龄大鼠耳蜗破坏后相比，3月龄大鼠慢性听觉剥夺后出现的显著差异较少。摄取量减少与报道的细胞形态变化相对应，听觉剥夺后未成熟大鼠的细胞形态变化也比成熟大鼠更大。结果表明，棕榈酸酯法可用于识别感觉剥夺后大脑脂质更新的长期区域变化。
