Yamazaki S, Noronha J G, Bell J M, Rapoport S I
Laboratory of Neurosciences, National Institute on Aging, Bethesda, MD 20892.
Brain Res. 1989 Jan 16;477(1-2):19-28. doi: 10.1016/0006-8993(89)91390-5.
Incorporation of plasma [14C]palmitate into the hypoglossal nucleus of the rat following transection, with and without regeneration of the hypoglossal nerve, was studied using quantitative autoradiography. The left hypoglossal nerve of 3-month-old, male Fischer-344 rats was transected using either: (1) an R-operation, which allowed nerve regeneration; or (2) a D-operation, in which regeneration was prevented. One to 84 days after axotomy, [14C]palmitate was injected intravenously and its rates of incorporation into stable structures of the left and right hypoglossal nuclei were measured at 4 h after injection. Following the R-operation, incorporation into the left hypoglossal nucleus was increased during and following axonal regeneration (up to 23% compared to control side), whereas incorporation was decreased 6-7% in the absence of regeneration, using the D-operation. The time courses of incorporation in both cases corresponded to histological changes, especially cell membrane changes following axotomy and suggest that [14C]palmitate incorporation reflects regenerative and degenerative neuronal changes associated with changes in lipid synthesis.
采用定量放射自显影术，研究了切断舌下神经后，在有或无舌下神经再生的情况下，血浆[14C]棕榈酸酯掺入大鼠舌下神经核的情况。对3个月大的雄性Fischer-344大鼠的左侧舌下神经进行切断，采用以下两种方法之一：（1）R手术，可使神经再生；或（2）D手术，阻止再生。轴突切断后1至84天，静脉注射[14C]棕榈酸酯，并在注射后4小时测量其掺入左右舌下神经核稳定结构的速率。采用R手术时，在轴突再生期间及之后，掺入左侧舌下神经核的量增加（与对照侧相比增加高达23%），而采用D手术在无再生的情况下，掺入量减少6-7%。两种情况下掺入的时间进程与组织学变化相对应，特别是轴突切断后的细胞膜变化，这表明[14C]棕榈酸酯的掺入反映了与脂质合成变化相关的再生和退化性神经元变化。
