Ten Kah Ern, Md Zoqratt Muhammad Zarul Hanifah, Ayub Qasim, Tan Hock Siew
School of Science, Monash University Malaysia, 47500, Bandar Sunway, Selangor Darul Ehsan, Malaysia.
Monash University Malaysia Genomics Facility, 47500, Bandar Sunway, Selangor Darul Ehsan, Malaysia.
BMC Res Notes. 2021 Mar 4;14(1):83. doi: 10.1186/s13104-021-05493-z.
The nosocomial pathogen, Acinetobacter baumannii, has acquired clinical significance due to its ability to persist in hospital settings and survive antibiotic treatment, which eventually resulted in the rapid spread of this bacterium with antimicrobial resistance (AMR) phenotypes. This study used a multidrug-resistant A. baumannii (strain ATCC BAA1605) as a model to study the genomic features of this pathogen.
One circular chromosome and one circular plasmid were discovered in the complete genome of A. baumannii ATCC BAA1605 using whole-genome sequencing. The chromosome is 4,039,171 bp long with a GC content of 39.24%. Many AMR genes, which confer resistance to major classes of antibiotics (beta-lactams, aminoglycosides, tetracycline, sulphonamides), were found on the chromosome. Two genomic islands were predicted on the chromosome, one of which (Genomic Island 1) contains a cluster of AMR genes and mobile elements, suggesting the possibility of horizontal gene transfer. A subtype I-F CRISPR-Cas system was also identified on the chromosome of A. baumannii ATCC BAA1605. This study provides valuable genome data that can be used as a reference for future studies on A. baumannii. The genome of A. baumannii ATCC BAA1605 has been deposited at GenBank under accession no. CP058625 and CP058626.
医院病原菌鲍曼不动杆菌因其能在医院环境中持续存在并在抗生素治疗下存活，从而具有了临床意义，这最终导致该细菌携带抗菌耐药（AMR）表型迅速传播。本研究以一株多重耐药鲍曼不动杆菌（菌株ATCC BAA1605）为模型，研究该病原菌的基因组特征。
通过全基因组测序，在鲍曼不动杆菌ATCC BAA1605的完整基因组中发现了一条环状染色体和一个环状质粒。该染色体长度为4,039,171 bp，GC含量为39.24%。在染色体上发现了许多赋予对主要抗生素类别（β-内酰胺类、氨基糖苷类、四环素、磺胺类）耐药性的AMR基因。在染色体上预测到两个基因组岛，其中一个（基因组岛1）包含一组AMR基因和移动元件，提示存在水平基因转移的可能性。在鲍曼不动杆菌ATCC BAA1605的染色体上还鉴定出一个I-F型CRISPR-Cas系统。本研究提供了有价值的基因组数据，可作为未来鲍曼不动杆菌研究的参考。鲍曼不动杆菌ATCC BAA1605的基因组已保存在GenBank，登录号为CP058625和CP058626。
