Tesfamariam B, Halpern W
Department of Physiology and Biophysics, University of Vermont, College of Medicine, Burlington.
Hypertension. 1988 May;11(5):440-4. doi: 10.1161/01.hyp.11.5.440.
The endothelium-dependent and presumed endothelium-independent vasodilators acetylcholine and sodium nitroprusside, respectively, were used to characterize relaxation responses of mesenteric resistance arteries from stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar-Kyoto rats (WKY). Vessels were preconstricted using concentrations of norepinephrine or 5-hydroxytryptamine, which reduced their diameters by 50 to 60%. Relaxation responses to acetylcholine (10(-8) - 10(-7) M) were significantly smaller (p less than 0.05) in vessel segments from SHRSP, but the maximal relaxations at higher concentrations were the same in both strains. However, SHRSP vessels relaxed to a greater extent than did those of the WKY at all concentrations of sodium nitroprusside. Endothelium removal significantly enhanced sodium nitroprusside-induced dilations in both rat strains, and the dilations were significantly greater in segments from SHRSP in the concentration range of 3 X 10(-8) to 10(-6) M. The decreased relaxation to acetylcholine in resistance arteries from adult hypertensive rats compared with those from the normotensive strain suggests that functional alterations in the endothelium may play a role in hypertensive disease.
分别使用内皮依赖性且推测为非内皮依赖性的血管舒张剂乙酰胆碱和硝普钠，来表征易患中风的自发性高血压大鼠（SHRSP）和Wistar-Kyoto大鼠（WKY）肠系膜阻力动脉的舒张反应。使用去甲肾上腺素或5-羟色胺浓度使血管预先收缩，使血管直径缩小50%至60%。SHRSP血管段对乙酰胆碱（10^(-8) - 10^(-7) M）的舒张反应明显较小（p < 0.05），但在较高浓度下两种品系的最大舒张程度相同。然而，在所有硝普钠浓度下，SHRSP血管的舒张程度均比WKY血管更大。去除内皮显著增强了两种大鼠品系中硝普钠诱导的舒张，并且在3×10^(-8)至10^(-6) M浓度范围内，SHRSP血管段的舒张明显更大。与正常血压品系相比，成年高血压大鼠阻力动脉对乙酰胆碱的舒张反应降低，这表明内皮功能改变可能在高血压疾病中起作用。
