Castro Antonio Jesús, Lima-Cabello Elena, Alché Juan de Dios
Plant Reproductive Biology and Advanced Imaging Laboratory, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín (CSIC), 18008 Granada, Spain.
Data Brief. 2021 Jan 30;35:106822. doi: 10.1016/j.dib.2021.106822. eCollection 2021 Apr.
Proteins and peptides are minor components of vegetal oils. The presence of these compounds in virgin olive oil was first reported in 2001, but the nature of the olive oil proteome is still a puzzling question for food science researchers. In this paper, we have compiled for a first time a comprehensive proteomic dataset of olive fruit and fungal proteins that are present at low but measurable concentrations in a vegetable oil from a crop of great agronomical relevance as olive ( L.). Accurate mass nLC-MS data were collected in high definition direct data analysis (HD-DDA) mode using the ion mobility separation step. Protein identification was performed using the Mascot Server v2.2.07 software (Matrix Science) against an ad hoc database made of olive protein entries. Starting from this proteomic record, the impact of these proteins on olive oil stability and quality could be tested. Moreover, the effect of olive oil proteins on human health and their potential use as functional food components could be also evaluated. In addition, this dataset provides a resource for use in further functional comparisons across other vegetable oils, and also expands the proteomic resources to non-model species, thus also allowing further comparative inter-species studies. The data presented here are related to the research article of Castro et al. [1].
蛋白质和肽是植物油中的微量成分。2001年首次报道了初榨橄榄油中存在这些化合物,但橄榄油蛋白质组的性质对于食品科学研究人员来说仍然是一个令人困惑的问题。在本文中,我们首次汇编了一个全面的蛋白质组数据集,该数据集包含了橄榄果实和真菌蛋白质,这些蛋白质在具有重大农业意义的作物(如油橄榄(L.))的植物油中以低但可测量的浓度存在。使用离子淌度分离步骤,以高清直接数据分析(HD-DDA)模式收集了精确质量的纳升液相色谱-质谱数据。使用Mascot Server v2.2.07软件(Matrix Science)针对由橄榄蛋白条目组成的特定数据库进行蛋白质鉴定。从这个蛋白质组记录出发,可以测试这些蛋白质对橄榄油稳定性和质量的影响。此外,还可以评估橄榄油蛋白质对人类健康的影响及其作为功能性食品成分的潜在用途。此外,该数据集为跨其他植物油进行进一步的功能比较提供了资源,还将蛋白质组资源扩展到非模式物种,从而也允许进行进一步的种间比较研究。这里呈现的数据与Castro等人的研究文章[1]相关。