Fain G L, Cilluffo M C, Fain M J, Lee D A
Department of Ophthalmology, Jules Stein Eye Institute, UCLA School of Medicine 90024-1771.
Invest Ophthalmol Vis Sci. 1988 May;29(5):817-21.
We describe a new technique for the separation and isolation of nonpigmented ciliary body epithelial cells from rabbit. Excised ciliary body is incubated in a medium buffered with EGTA to a free-Ca2+ concentration of 10(-8) M, and the nonpigmented cell layer is separated from the pigmented layer by microdissection under direct visualization. This technique yields intact cells which are greater than 99% nonpigmented. It can be used to produce preparations of nonpigmented cell plasma membrane and of viable whole cells, which may be useful for biochemistry, pharmacology, cell transport studies and tissue culture.
我们描述了一种从兔子中分离和纯化无色素睫状体上皮细胞的新技术。将切除的睫状体在含有EGTA缓冲的培养基中孵育，使游离钙离子浓度达到10（-8）M，然后在直接观察下通过显微切割将无色素细胞层与色素层分离。该技术可产生完整的细胞，其中无色素细胞大于99%。它可用于制备无色素细胞质膜和活的全细胞制剂，这可能对生物化学、药理学、细胞转运研究和组织培养有用。
