Fain G L, Smolka A, Cilluffo M C, Fain M J, Lee D A, Brecha N C, Sachs G
Department of Ophthalmology, Jules Stein Eye Institute, University of California School of Medicine, Los Angeles 90024.
Invest Ophthalmol Vis Sci. 1988 May;29(5):785-94.
Monoclonal antibodies developed against the transport enzyme H+-K+ ATPase of gastric mucosa selectively bind to the nonpigmented cell layer in both the pars plana and pars plicata of the rabbit ciliary body epithelium. The most specific of these antibodies (HK 12.18) reacts with the nonpigmented cells but with no other cell type in ciliary body, retina or iris. Antibody reactivity is evinced by a distinct particulate staining which is greater on the surfaces of the nonpigmented cells than within the cytoplasm and seems most intense on the apical membrane between the pigmented and nonpigmented cell layers. This pattern of staining was present in 2-3-day-old pups and in young adults, in both pigmented and albino animals. Similar staining was not observed in control sections treated with preimmune serum, nonspecific mouse monoclonal antibody, or HK 12.18 preabsorbed to gastric microsomes. In addition to staining intact ciliary body, the antibody HK 12.18 can be shown to react with a population of cells lacking pigment granules in suspensions of dissociated ciliary body epithelium. This antibody may therefore be useful as a marker for the identification of nonpigmented cells in tissue culture.
针对胃黏膜转运酶H⁺-K⁺ATP酶研制的单克隆抗体，可选择性地与兔睫状体上皮扁平部和皱襞部的无色素细胞层结合。其中最具特异性的抗体（HK 12.18）与无色素细胞发生反应，但不与睫状体、视网膜或虹膜中的其他细胞类型发生反应。抗体反应表现为明显的颗粒状染色，在无色素细胞表面比在细胞质内更明显，并且在色素细胞层和无色素细胞层之间的顶端膜上似乎最为强烈。这种染色模式在2 - 3日龄幼崽和年轻成年动物中均有出现，包括有色和白化动物。在用免疫前血清、非特异性小鼠单克隆抗体或预先吸附到胃微粒体上的HK 12.18处理的对照切片中未观察到类似的染色。除了对完整的睫状体进行染色外，抗体HK 12.18还可显示与解离的睫状体上皮细胞悬液中一群缺乏色素颗粒的细胞发生反应。因此，这种抗体可能作为一种标志物用于在组织培养中鉴定无色素细胞。
