Gaál G, Bácsy E, Rappay G
Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest.
Histochemistry. 1988;88(3-6):401-6. doi: 10.1007/BF00570301.
Cultured cells from the anterior pituitary glands of adult rats were treated with the tripeptide aldehyde proteinase inhibitor, BOC-DPhe-Phe-Lys-H. The addition of this tripeptide aldehyde decreased the in vitro release of prolactin to 25% of the control value, while the release of growth hormone in the same cultures decreased to 33% of the control value. Prolactin immunostaining was stronger in semithin sections of proteinase-inhibitor-treated cultures than in control sections. After 2 h treatment with the inhibitor, prolactin- and growth hormone-containing secretory granules were numerous, and the number of crinophagic vacuoles had increased. In the presence of the inhibitor, the overall cytoarchitecture of parenchymal cells was well preserved, and the pathway of the uptake of cationic ferritin appeared to be unaffected.
用三肽醛蛋白酶抑制剂BOC-DPhe-Phe-Lys-H处理成年大鼠垂体前叶的培养细胞。添加这种三肽醛后，催乳素的体外释放量降至对照值的25%，而同一培养物中生长激素的释放量降至对照值的33%。在蛋白酶抑制剂处理的培养物的半薄切片中，催乳素免疫染色比对照切片更强。用抑制剂处理2小时后，含催乳素和生长激素的分泌颗粒数量众多，且吞饮空泡的数量增加。在抑制剂存在的情况下，实质细胞的整体细胞结构保存良好，阳离子铁蛋白的摄取途径似乎未受影响。
