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鉴定新型中间宿主白尾海雕(Haliaeetus albicilla)中似 wobeseri 新孢子虫的分子特征。

Molecular identification of Sarcocystis wobeseri-like parasites in a new intermediate host species, the white-tailed sea eagle (Haliaeetus albicilla).

机构信息

Institute of Zoology, Zoological Society of London, London, NW1 4RY, UK.

Finn Pathologists, Weybread, Suffolk, IP21 5TT, UK.

出版信息

Parasitol Res. 2021 May;120(5):1845-1850. doi: 10.1007/s00436-021-07103-0. Epub 2021 Mar 5.

DOI:10.1007/s00436-021-07103-0
PMID:33666756
Abstract

A reintroduced white-tailed sea eagle (Haliaeetus albicilla) in moderate body condition was found dead and submitted for post-mortem examination. There were no signs of disease on gross pathological examination. Histopathological examination however revealed the presence of encysted protozoan parasites in pectoral and cardiac muscle sections. Polymerase chain reaction amplification of extracted genomic DNA and sequencing of four regions: the 18S rDNA, 28S rDNA, internal transcribed spacer (ITS) 1, and RNA polymerase B (rpoB) loci, confirmed the presence of a Sarcocystis species in pectoral and cardiac muscle which appeared phylogenetically similar to Sarcocystis wobeseri. This is the first report of S. wobeseri-like infection in a white-tailed sea eagle revealing a new intermediate host species for this parasite.

摘要

一只身体状况中等的重新引入的白尾海雕(Haliaeetus albicilla)被发现死亡并被提交进行剖检。大体病理检查未见疾病迹象。然而,组织病理学检查显示在胸肌和心肌切片中存在包囊原生动物寄生虫。从提取的基因组 DNA 进行聚合酶链反应扩增,并对四个区域进行测序:18S rDNA、28S rDNA、内部转录间隔区(ITS)1 和 RNA 聚合酶 B(rpoB)基因座,证实胸肌和心肌中存在一种 Sarcocystis 物种,其系统发育与 Sarcocystis wobeseri 相似。这是首例在白尾海雕中发现的 S. wobeseri 样感染,表明该寄生虫有了新的中间宿主物种。

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