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壳聚糖纳米载卡介苗多糖核酸与卵清白蛋白对哮喘小鼠气道炎症的治疗作用。

Therapeutic efficacy of chitosan nanoparticles loaded with BCG-polysaccharide nucleic acid and ovalbumin on airway inflammation in asthmatic mice.

机构信息

Department of Respiration, The First Hospital of Huzhou, The First Affiliated Hospital of Huzhou University, No.158 Guang Changhou Road, Huzhou, 313000, Zhejiang, China.

Department of Intensive Care Unit, The First Hospital of Huzhou, The First Affiliated Hospital of Huzhou University, Huzhou, 313000, Zhejiang, China.

出版信息

Eur J Clin Microbiol Infect Dis. 2021 Aug;40(8):1623-1631. doi: 10.1007/s10096-021-04183-9. Epub 2021 Mar 5.

DOI:10.1007/s10096-021-04183-9
PMID:33666790
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7934352/
Abstract

In this study, immunoregulation and desensitization therapies were jointly applied in the treatment of asthma, in which chitosan (CS) nanoparticles were used. BALB/c mice were selected and mouse models of asthma were constructed. Mice were divided into 7 groups. A double-chamber plethysmograph, MTT, hematoxylin-eosin staining, and ELISA were used. The expression levels of IL-4 and IL-5 in lung tissue cells were detected. CS-BCG-PSN-OVA sustained-release vaccines significantly alleviated airway hyperresponsiveness (AHR) in asthmatic mice. The numbers of total lymphocytes and eosinophils in BALF were remarkably reduced. The expression levels of IL-4 and IL-5 in lung tissue cells of the treatment groups were dramatically decreased. CS-BCG-PSN-OVA was found in vitro to be able to inhibit OVA-induced T-cell proliferation and upregulate the proportion of CD4+CD25+Foxp3+ T cells. CS-BCG-PSN-OVA sustained-release vaccine could significantly attenuate AHR and airway inflammation in asthmatic mice. Thus, it has a promising application prospect for the treatment of bronchial asthma.

摘要

在这项研究中，采用壳聚糖（CS）纳米粒联合免疫调节和脱敏疗法治疗哮喘，选择 BALB/c 小鼠构建哮喘模型，将小鼠分为 7 组，采用双室肺量计、MTT、苏木精-伊红染色和 ELISA 法检测，检测肺组织细胞中白细胞介素-4（IL-4）和白细胞介素-5（IL-5）的表达水平。CS-BCG-PSN-OVA 缓释疫苗能显著减轻哮喘小鼠气道高反应性（AHR），显著减少 BALF 中总淋巴细胞和嗜酸性粒细胞的数量，降低肺组织细胞中 IL-4 和 IL-5 的表达水平。体外实验发现 CS-BCG-PSN-OVA 能抑制 OVA 诱导的 T 细胞增殖，上调 CD4+CD25+Foxp3+T 细胞比例。CS-BCG-PSN-OVA 缓释疫苗能显著减轻哮喘小鼠 AHR 和气道炎症，对于治疗支气管哮喘具有广阔的应用前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86cd/7934352/53f907b02d1e/10096_2021_4183_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86cd/7934352/ebc4d5ca393a/10096_2021_4183_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86cd/7934352/842837591625/10096_2021_4183_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86cd/7934352/c0fae7084ffd/10096_2021_4183_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86cd/7934352/7babc6f7555f/10096_2021_4183_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86cd/7934352/53f907b02d1e/10096_2021_4183_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86cd/7934352/ebc4d5ca393a/10096_2021_4183_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86cd/7934352/842837591625/10096_2021_4183_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86cd/7934352/c0fae7084ffd/10096_2021_4183_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86cd/7934352/7babc6f7555f/10096_2021_4183_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86cd/7934352/53f907b02d1e/10096_2021_4183_Fig5_HTML.jpg

相似文献

[1]
Therapeutic efficacy of chitosan nanoparticles loaded with BCG-polysaccharide nucleic acid and ovalbumin on airway inflammation in asthmatic mice.

Eur J Clin Microbiol Infect Dis. 2021-8

[2]
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[3]
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[5]
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[6]
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[7]
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[8]
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[9]
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[10]
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引用本文的文献

[1]
Treatment of lung diseases nanoparticles and nanorobots: Are these viable alternatives to overcome current treatments?

Mater Today Bio. 2025-2-26

[2]
Recent Advances in Nanomaterials for Asthma Treatment.

Int J Mol Sci. 2022-11-20

[3]
Multiple Roles of Chitosan in Mucosal Drug Delivery: An Updated Review.

Mar Drugs. 2022-5-20

本文引用的文献

[1]
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J Allergy Clin Immunol. 2010-2

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