针刺对 DHEA 诱导的多囊卵巢综合征模型大鼠卵巢形态和功能的影响。
Effect of Acupuncture on Ovary Morphology and Function in DHEA-Induced Polycystic Ovary Syndrome Model Rats.
机构信息
Department of Acupuncture, the Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing o100029, China.
Graduate School, Beijing University of Chinese Medicine, Beijing, 100029, China.
出版信息
Chin J Integr Med. 2021 Mar;27(3):220-224. doi: 10.1007/s11655-021-3290-0. Epub 2021 Mar 5.
OBJECTIVE
To investigate the effects of acupuncture on ovary morphology and function in dehydroepiandrosterone (DHEA)-induced polycystic ovary syndrome (PCOS) model rats.
METHODS
A total of 40 adult female Wistar rats were randomly allocated to 4 groups by a random number table, including control, model, metformin and acupuncture groups, 10 rats in each group. PCOS rat model was developed by injecting with DHEA (6 mg/100 g body weight) in 0.2 mL of oil subcutaneously. Electrical stimulation (2 Hz, 3 mA) was applied to Guanyuan (CV 4), Zigong (EX-CA1) and Qihai (CV 6) acupoints for 30 min daily in the acupuncture group, and metformin (200 mg/kg) was given to rats in the metformin group, both once per day for 21 consecutive days, and rats in the normal group was fed with normal saline and fed regularly. After 21 days of administration, the rat blood samples were collected for detecting the reproductive hormonal levels [luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E), progesterone (P), testosterone (T)] and inflammatory factors (visfatin, IL-6) analysis. Ovary tissue was used for histopathological analysis.
RESULTS
Compared with the model group, rats in the acupuncture and metformin groups were significantly lower in weight gain, FSH, LH and T levels, and E and P levels significantly increased (alll P<0.05). Meanwhile, LH and FSH levels were significantly decreased, and P, T and E levels significantly increased in the acupuncture group, compared with the metformin group (P<0.05). Compared with the model group, IL-6 and visfatin levels were significantly decreased in the acupuncture and metformin groups (P<0.05). There were no significant differences in IL-6 and visfatin levels between the acupuncture and metformin groups (P>0.05). Ovarian diameter in the acupuncture and metformin groups were smaller than the model group (P<0.05). However, there were no significant differences in ovarian diameters between the acupuncture and metformin groups (P>0.05).
CONCLUSION
Acupuncture might improve ovary morphology and its function in DHEA-induced PCOS model rats.
目的
探讨针刺对脱氢表雄酮（DHEA）诱导多囊卵巢综合征（PCOS）模型大鼠卵巢形态和功能的影响。
方法
将 40 只成年雌性 Wistar 大鼠按随机数字表法分为 4 组，每组 10 只，分别为空白对照组、模型组、二甲双胍组和针刺组。采用 DHEA（6mg/100g 体重）0.2mL 油溶液皮下注射法建立 PCOS 大鼠模型。针刺组每日针刺关元（CV4）、子宫（EX-CA1）和气海（CV6）穴，电流强度 2Hz、3mA，持续 30min；二甲双胍组给予二甲双胍（200mg/kg）灌胃，每日 1 次，连续 21d；空白对照组给予等容量生理盐水灌胃，常规饲养。给药 21d 后，采集大鼠血样，检测生殖激素水平[促黄体生成素（LH）、促卵泡生成素（FSH）、雌二醇（E）、孕酮（P）、睾酮（T）]和炎症因子（内脂素、IL-6）水平，取卵巢组织进行组织病理学分析。
结果
与模型组比较，针刺组和二甲双胍组大鼠体质量增长缓慢，FSH、LH、T 水平降低，E、P 水平升高（均 P<0.05）；针刺组 LH、FSH 水平降低，P、T、E 水平升高，与二甲双胍组比较差异有统计学意义（P<0.05）。与模型组比较，针刺组和二甲双胍组 IL-6、内脂素水平降低（P<0.05）。两组 IL-6、内脂素水平比较差异无统计学意义（P>0.05）。针刺组和二甲双胍组大鼠卵巢直径小于模型组（P<0.05）。但两组大鼠卵巢直径比较差异无统计学意义（P>0.05）。
结论
针刺可能通过改善 DHEA 诱导 PCOS 模型大鼠卵巢形态和功能。
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