Zhang Wei-yi, Huang Guang-ying, Liu Jie
Institute of Integrated Traditional Chinese and Western Medicine, Tongfi Hospital, Tongii Medical College, Huazhong University of Science and Technology, Wuhan.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2009 Nov;29(11):997-1000.
To observe the effect and mechanism of acupuncture on infertility of rats with polycystic ovarian syndrome (PCOS).
PCOS rat model was induced by subcutaneous injection of oil solution of dehydroepiandrosterone (DHEA) in immature (24-day-old) female rats for continuous 20 days. Rats for control were given with same dose of oil for instead. PCOS rats were randomly divided into the model group untreated and the acupuncture group treated by needling acupoints of Guanyuan (CV4), Zhongji (CV3), Sanyin-jiao (SP6) and Zigong (CX-CA1), 15 min once a day for 5 continuous days, starting from the 80th day after birth. All rats were sacrificed at terminal of the treatment, their uterus and bilateral ovaries were dissected for observation and blood levels of sex hormones were measured.
compared with the model group, the number of implanted blastocyte and blastocyte implantation rate were higher and the blood levels of testosterone (T) and estradiol (E2) were lower in the acupuncture group (P < 0.05); but the difference between groups in serum levels of follicular stimulating hormone, luteinizing hormone and progesterone were of statistical insignificance (P > 0.05). Moreover, the wet weight of ovary was lower and the equipotent diameter and area of glandular organ and cavity, area ratio of gland and the stroma, and mean thickness of endometria were higher in the acupuncture group than those in the control group (P < 0.05).
Acupuncture can conspicuously downregulate the expressions of serum levels of T and E2, improve the development of ovaries and uterus, promote ovulation, enhance endometrial receptivity, and advance blastocyte implantation.
观察针刺对多囊卵巢综合征（PCOS）不孕大鼠的影响及作用机制。
将脱氢表雄酮（DHEA）油溶液皮下注射于未成熟（24日龄）雌性大鼠，连续20天，诱导建立PCOS大鼠模型。对照组大鼠注射相同剂量的油剂。将PCOS大鼠随机分为未治疗的模型组和针刺组，针刺组针刺关元（CV4）、中极（CV3）、三阴交（SP6）和子宫（CX - CA1）穴位，每天1次，每次15分钟，连续5天，从出生后第80天开始。治疗结束后处死所有大鼠，解剖子宫和双侧卵巢进行观察，并检测性激素水平。
与模型组相比，针刺组着床胚泡数和胚泡着床率较高，睾酮（T）和雌二醇（E2）血水平较低（P < 0.05）；但两组间促卵泡生成素、黄体生成素和孕酮血清水平差异无统计学意义（P > 0.05）。此外，针刺组卵巢湿重较低，腺体器官和腔隙的等效直径和面积、腺体与基质面积比以及子宫内膜平均厚度均高于模型组（P < 0.05）。
针刺可显著下调血清T和E2水平的表达，改善卵巢和子宫发育，促进排卵，增强子宫内膜容受性，促进胚泡着床。
