Tirado Thais Cristina, Bavia Lorena, Ambrosio Altair Rogerio, Campos Monique Paiva, de Almeida Santiago Marta, Messias-Reason Iara Jose, Figueiredo Fabiano Borges
Laboratório de Biologia Celular, Instituto Carlos Chagas, Fundação Oswaldo Cruz (FIOCruz), Curitiba, Paraná, Brazil.
Laboratório de Imunopatologia Molecular, Departamento de Patologia Médica, Universidade Federal Do Paraná (UFPR), Curitiba, Paraná, Brazil.
Dev Comp Immunol. 2021 Jul;120:104061. doi: 10.1016/j.dci.2021.104061. Epub 2021 Mar 3.
Visceral Leishmaniasis is an infectious disease that affects mainly humans and dogs, with the latter being important reservoirs of the parasite. Conversely, cats are naturally resistant. The immune system can offer important explanation to this problematic as there is no evidence on the role that the complement system plays in cats. In this context, effect of the complement system from human, dog and cat sera on Leishmania infantum was evaluated. Activation of the classical, alternative and lectin pathways was assessed through hemolytic and ELISA assays. Lytic activity of the complement on the parasite's viability was investigated by Transmission Electron Microscopy and Flow Cytometry. Complement proteins were more consumed in dog serum on the classical and alternative pathways, leading to less hemolytic activity, and only in cat serum they were consumed on the lectin pathway when incubated with L. infantum. Lytic activity on the parasite's surface was more accentuated in human serum, and varied throughout the parasite's developmental stages.
内脏利什曼病是一种主要影响人类和犬类的传染病，犬类是该寄生虫的重要宿主。相反，猫具有天然抗性。免疫系统可以为这一问题提供重要解释，因为尚无证据表明补体系统在猫中发挥的作用。在此背景下，评估了人、犬和猫血清中的补体系统对婴儿利什曼原虫的影响。通过溶血和酶联免疫吸附测定评估经典途径、替代途径和凝集素途径的激活情况。通过透射电子显微镜和流式细胞术研究补体对寄生虫活力的溶解活性。在经典途径和替代途径中，犬血清中的补体蛋白消耗更多，导致溶血活性降低，而在与婴儿利什曼原虫孵育时，仅猫血清中的补体蛋白在凝集素途径中被消耗。人血清中对寄生虫表面的溶解活性更为明显，并且在寄生虫的整个发育阶段有所不同。
