Colegio de Postgraduados, Campus Montecillo, Estado de México, Mexico; Escuela Superior de Ciencias Agropecuarias. Universidad Autónoma de Campeche, Mexico.
Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, INIFAP, Jiutepec, Morelos, Mexico.
Vet Immunol Immunopathol. 2021 Apr;234:110218. doi: 10.1016/j.vetimm.2021.110218. Epub 2021 Feb 25.
The immune response and phenotypic characteristics of Pelibuey lambs were analysed after the induction of a Haemonchus contortus trickle infection. Male lambs (n = 29; 20 kg live weight) were infected with 100 H. contortus infective larvae per kg of live weight on day 3, 5 and 7 of the experiment. The number of eggs per gram (epg), seven haematological parameters and the immunoglobulin A (IgA) level were analysed for 56 experimental days. In addition, histopathological samples from the fundic abomasal region and the relative expression of 10 immune-related genes from 15 infected and three non-infected lambs were analysed at day 0 and 49 of the experiment. The epg count and some haematological parameters (leucocytes, red blood cells, haemoglobin and total protein) with statistically significant differences (P < 0.01) were used to identify nine resistant and 20 susceptible lambs (1166 ± 1071 and 3171 ± 1463 epg, respectively). Moreover, acute infiltration of immune cells and parasitic granuloma formation were observed in susceptible lambs; the resistant group had moderate inflammatory cell infiltration. With respect to relative gene expression, resistant lambs showed upregulation (P < 0.001) of 10 genes, from 2.2 to 15.99 fold. Moreover, there was a strong indirect correlation (P < 0.05) between the epg count and interleukin 5 (IL5) gene expression. By contrast, there was an average 0.34 fold downregulation in nine of the immune-related genes (P ≤ 0.05) in susceptible lambs (the only exception was Fc fragment of IgE receptor Ia [FCER1A] upregulation). In addition, there was a direct correlation (P ≤ 0.05) between the epg count and the expression of IL8, which encodes an inflammatory chemokine. In conclusion, this study showed differential IL5 and IL8 gene expression during haemonchosis in resistant and susceptible Pelibuey lambs, respectively, together with a variable immune response based on histopathological and haematological parameters.
在诱导 Pelibuey 羔羊感染捻转血矛线虫滴注感染后，分析了其免疫反应和表型特征。雄性羔羊（n=29；20kg 活重）在实验第 3、5 和 7 天，每公斤活重感染 100 条捻转血矛线虫感染性幼虫。在 56 个实验日分析了每克粪便虫卵数（epg）、7 项血液参数和免疫球蛋白 A（IgA）水平。此外，在实验第 0 天和第 49 天，还分析了胃底皱胃区的组织病理学样本以及 15 只感染和 3 只非感染羔羊的 10 个免疫相关基因的相对表达。epg 计数和一些具有统计学显著差异（P<0.01）的血液参数（白细胞、红细胞、血红蛋白和总蛋白）用于鉴定 9 只抗性和 20 只敏感性羔羊（分别为 1166±1071 和 3171±1463 epg）。此外，在敏感性羔羊中观察到免疫细胞的急性浸润和寄生性肉芽肿形成；抗性组有中度炎症细胞浸润。关于相对基因表达，抗性羔羊的 10 个基因表达上调（P<0.001），上调幅度为 2.2 至 15.99 倍。此外，epg 计数与白细胞介素 5（IL5）基因表达之间存在强的间接相关性（P<0.05）。相比之下，在敏感性羔羊中，9 个免疫相关基因的表达平均下调 0.34 倍（唯一的例外是 IgE 受体 Ia 的 Fc 片段[FCER1A]上调）（P≤0.05）。此外，epg 计数与编码炎症趋化因子的白细胞介素 8（IL8）的表达之间存在直接相关性（P≤0.05）。总之，本研究表明，在抗性和敏感性 Pelibuey 羔羊的捻转血矛线虫感染过程中，IL5 和 IL8 基因的表达存在差异，同时基于组织病理学和血液参数的免疫反应存在差异。
