Facultad de Ingenieros en Biotecnología, Universidad Politécnica del Estado de Morelos, Jiutepec, Morelos, Mexico.
Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Carr. Fed. Cuernavaca-Cuautla No. 8254, Jiutepec, Morelos C.P. 62550, Mexico.
Vet Parasitol Reg Stud Reports. 2020 Jul;21:100422. doi: 10.1016/j.vprsr.2020.100422. Epub 2020 Jun 13.
We analysed the immune response involved in sheep naturally infected with gastrointestinal (GI) nematodes. Fifteen Pelibuey lambs were grazed in paddocks contaminated with GI nematodes for 13 weeks. To assess the infection, the number of eggs per gram (epg) and the percentage of packed cell volume (pcv) were evaluated. Blood and abomasal tissue samples were collected at week 8 post-infection to analyse the expression levels of IL-4, IL-5, IL-6, IL-8, IL-13, TGF-β and FCεR1A genes. The nematode Haemonchus contortus was the main species identified. In addition, two groups of lambs were classified based on the x ± SE of epg and pcv values: G-1, with 151 ± 28 and 29 ± 0.33%, respectively, and G-2, with 475 ± 59.5 and 26 ± 0.38%, respectively. For G-1, upregulation of IL-4, IL-8, IL-13, TGF-β and FCεR1A genes from 2.42- to 14.99-fold was observed in blood and abomasal tissue samples (p > .05), and IL-5, IL-8 and TGF-β genes had significant gene expression levels in blood (p < .05). For G-2, moderate gene expression levels, ranging from 1.22- to 3.45-fold, were observed in abomasal tissue (p > .05), and the IL-5 gene presented significant gene expression in blood (p < .05). Strong positively correlated values (r) between pcv and IL-4, IL-8 and TGF-β genes were observed in G-1. In contrast, significant negative correlations between epg and IL-4, IL-5 and FCεR1A genes indicate acute infection for G-2. Our results suggest that IL-4, IL-5, IL-13, TGF-β and FCεR1A genes are important modulators of GI nematode infections of Pelibuey lambs.
我们分析了绵羊自然感染胃肠道（GI）线虫所涉及的免疫反应。15 只佩里布尤羔羊在被 GI 线虫污染的围场中放牧 13 周。为了评估感染情况，评估了每克粪便中的卵数（epg）和红细胞压积（pcv）的百分比。在感染后第 8 周采集血液和皱胃组织样本，以分析 IL-4、IL-5、IL-6、IL-8、IL-13、TGF-β 和 FCεR1A 基因的表达水平。鉴定出的主要线虫种是捻转血矛线虫。此外，根据 epg 和 pcv 值的 x ± SE 将两组羔羊分类：G-1，分别为 151 ± 28 和 29 ± 0.33%，G-2，分别为 475 ± 59.5 和 26 ± 0.38%。对于 G-1，在血液和皱胃组织样本中观察到 IL-4、IL-8、IL-13、TGF-β 和 FCεR1A 基因的上调，幅度为 2.42-14.99 倍（p>.05），并且 IL-5、IL-8 和 TGF-β 基因在血液中有显著的基因表达水平（p<.05）。对于 G-2，在皱胃组织中观察到适度的基因表达水平，范围为 1.22-3.45 倍（p>.05），并且 IL-5 基因在血液中具有显著的基因表达水平（p<.05）。在 G-1 中观察到 pcv 与 IL-4、IL-8 和 TGF-β 基因之间呈强正相关（r）。相比之下，epg 与 IL-4、IL-5 和 FCεR1A 基因之间存在显著负相关，表明 G-2 存在急性感染。我们的结果表明，IL-4、IL-5、IL-13、TGF-β 和 FCεR1A 基因是 Pelibuey 羔羊胃肠道线虫感染的重要调节因子。
