Fajstavrová Klára, Rimpelová Silvie, Fajstavr Dominik, Švorčík Václav, Slepička Petr
Department of Solid State Engineering, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic.
Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic.
Materials (Basel). 2021 Feb 13;14(4):889. doi: 10.3390/ma14040889.
The development of new biocompatible polymer substrates is still of interest to many research teams. We aimed to combine a plasma treatment of fluorinated ethylene propylene (FEP) substrate with a technique of improved phase separation. Plasma exposure served for substrate activation and modification of surface properties, such as roughness, chemistry, and wettability. The treated FEP substrate was applied for the growth of a honeycomb-like pattern from polystyrene solution. The properties of the pattern strongly depended on the primary plasma exposure of the FEP substrate. The physico-chemical properties such as changes of the surface chemistry, wettability, and morphology of the prepared pattern were determined. The cell response of primary fibroblasts and osteoblasts was studied on a honeycomb pattern. The prepared honeycomb-like pattern from polystyrene showed an increase in cell viability and a positive effect on cell adhesion and proliferation for both primary fibroblasts and osteoblasts.
新型生物相容性聚合物基底的开发仍然受到许多研究团队的关注。我们旨在将氟化乙烯丙烯（FEP）基底的等离子体处理与改进的相分离技术相结合。等离子体暴露用于基底活化以及表面性质（如粗糙度、化学性质和润湿性）的改性。经处理的FEP基底用于从聚苯乙烯溶液中生长出蜂窝状图案。该图案的性质强烈依赖于FEP基底的初始等离子体暴露。测定了所制备图案的物理化学性质，如表面化学变化、润湿性和形态。在蜂窝状图案上研究了原代成纤维细胞和成骨细胞的细胞反应。由聚苯乙烯制备的蜂窝状图案显示出细胞活力增加，并且对原代成纤维细胞和成骨细胞的细胞黏附和增殖均有积极影响。
