Sano T, Kudo H, Sugimoto T, Shikata E
Department of Botany, Hokkaido University, Sapporo, Japan.
J Virol Methods. 1988 Feb;19(2):109-19. doi: 10.1016/0166-0934(88)90154-1.
Four species of synthetic oligonucleotide probes for the diagnosis of hop stunt viroid (HSV) and citrus exocortis viroid (CEV) were devised. Probe HSV-1 detected all the members of HSV group, such as HSV-hop, HSV-grapevine, HSV-cucumber, HSV-citrus and a viroid-like RNA isolated from plum trees affected by plum dapple fruit disease. Probe HSV-2 discriminated HSV-grapevine from the other members of HSV group. HSV-hop and HSV-grapevine consist of the same numbers of nucleotides, with only one nucleotide exchange. It was also shown that the two viroids were indistinguishable by their biological and physicochemical properties. However, by using probe HSV-2, HSV-hop and HSV-grapevine were apparently differentiated. Probe CEV-1 detected all the members of potato spindle tuber viroid (PSTV) group, such as PSTV, CEV and chrysanthemum stunt viroid (CSV). Probe CEV-2 discriminated CEV from the other members of PSTV group. It is thus emphasized that synthetic oligonucleotide probes are useful for the diagnosis of viroids and their related strains. It was discussed that the method can be used for the diagnosis of viruses and their related strains.
设计了四种用于诊断啤酒花矮化类病毒（HSV）和柑橘裂皮类病毒（CEV）的合成寡核苷酸探针。探针HSV - 1能检测到HSV组的所有成员，如啤酒花HSV、葡萄HSV、黄瓜HSV、柑橘HSV以及从患李斑驳果病的李树中分离出的一种类病毒样RNA。探针HSV - 2能将葡萄HSV与HSV组的其他成员区分开来。啤酒花HSV和葡萄HSV的核苷酸数量相同，仅存在一个核苷酸的交换。研究还表明，这两种类病毒在生物学和物理化学性质上无法区分。然而，通过使用探针HSV - 2，啤酒花HSV和葡萄HSV能够明显区分开来。探针CEV - 1能检测到马铃薯纺锤块茎类病毒（PSTV）组的所有成员，如PSTV、CEV和菊花矮化类病毒（CSV）。探针CEV - 2能将CEV与PSTV组的其他成员区分开来。因此强调了合成寡核苷酸探针对于类病毒及其相关菌株的诊断是有用的。还讨论了该方法可用于病毒及其相关菌株的诊断。
