Haseloff J, Symons R H
Nucleic Acids Res. 1981 Jun 25;9(12):2741-52. doi: 10.1093/nar/9.12.2741.
The sequence of the 356 nucleotide residues of chrysanthemum stunt viroid (CSV) has been determined. Overlapping linear viroid fragments were obtained by partial ribonuclease digestion, radiolabelled in vitro at their 5'-ends, and sequenced using partial enzymic cleavage methods. Of the CSV sequence, 69% is contained in the published sequence of potato spindle tuber viroid (PSTV). Differences in the primary sequence of CSV and PSTV suggest that neither the positive nor putative negative strands of these two viroids code for functional polypeptide products. However, the two viroids can form similar secondary structures, implicating a role for viroid structure in replication.
已确定菊花矮缩类病毒（CSV）356个核苷酸残基的序列。通过部分核糖核酸酶消化获得重叠的线性类病毒片段，在体外对其5′端进行放射性标记，并使用部分酶切方法进行测序。CSV序列中，69%包含在已发表的马铃薯纺锤块茎类病毒（PSTV）序列中。CSV和PSTV一级序列的差异表明，这两种类病毒的正链或推定的负链均不编码功能性多肽产物。然而，这两种类病毒可形成相似的二级结构，这意味着类病毒结构在复制中起作用。
