Hammond R, Smith D R, Diener T O
Microbiology and Plant Pathology Laboratory, USDA-ARS Beltsville, MD 20705.
Nucleic Acids Res. 1989 Dec 11;17(23):10083-94. doi: 10.1093/nar/17.23.10083.
The Columnea latent viroid (CLV) occurs latently in certain Columnea erythrophae plants grown commercially. In potato and tomato, CLV causes potato spindle tuber viroid (PSTV)-like symptoms. Its nucleotide sequence and proposed secondary structure reveal that CLV consists of a single-stranded circular RNA of 370 nucleotides which can assume a rod-like structure with extensive base-pairing characteristic of all known viroids. The electrophoretic mobility of circular CLV under nondenaturing conditions suggests a potential tertiary structure. CLV contains extensive sequence homologies to the PSTV group of viroids but contains a central conserved region identical to that of hop stunt viroid (HSV). CLV also shares some biological properties with each of the two types of viroids. Most probably, CLV is the result of intracellular RNA recombination between an HSV-type and one or more PSTV-type viroids replicating in the same plant.
哥伦比亚潜隐类病毒（CLV）潜伏于某些商业化种植的红苞喜林芋属植物中。在马铃薯和番茄中，CLV会引发类似马铃薯纺锤块茎类病毒（PSTV）的症状。其核苷酸序列和推测的二级结构表明，CLV由一条370个核苷酸的单链环状RNA组成，该RNA可呈现出具有所有已知类病毒广泛碱基配对特征的棒状结构。在非变性条件下，环状CLV的电泳迁移率表明其具有潜在的三级结构。CLV与PSTV类病毒组存在广泛的序列同源性，但包含一个与啤酒花矮化类病毒（HSV）相同的中央保守区域。CLV还与这两类类病毒各自具有一些生物学特性。很可能，CLV是在同一植物中复制的HSV型和一种或多种PSTV型类病毒之间细胞内RNA重组的结果。
