Intracellular Microbiome Profiling of the Clinical Isolates from Lens Associated Keratitis.

act as hosts for various microorganisms and pathogens, causing Keratitis (AK). To investigate the association between endosymbionts and AK progression, we performed a metagenomics study to characterize the intracellular microbiome from five lenses associated with AK isolates and standard strains to characterize the role of ocular flora in AK progression. The used clinical isolates were axenic cultured from lenses associated with AK patients. AK isolates and standard controls such as 16S ribosomal RNA sequencing techniques were used for analysis. The microbiome compositions and relative abundance values were compared. The orders of and presented major populations of intracellular microbes belonging to all isolates. Comparison of the different source isolates showed that most of the abundance in keratitis isolates came from (121.0 folds), (54.15 folds), (24.51 folds), and (3.15 folds). Further analysis of the relative abundance data from keratitis isolates showed that was positively correlated with the disease course. In contrast, was found to be abundant in early-stage keratitis isolates. This study reveals the abundant anaerobic Gram-positive rods present in severe keratitis isolate and characterize the association between and ocular flora in AK progression.