MOE Key Lab of Environment and Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Int J Environ Res Public Health. 2021 Feb 16;18(4):1900. doi: 10.3390/ijerph18041900.
The effects of microcystin-RR (MC-RR) on water metabolism were studied on Sprague-Dawley (SD) rats and KunMing (KM) mice. In the single dose toxicity test, polydipsia, polyuria, hematuria and proteinuria were found in group of rats receiving a MC-RR dose of 574.7 μg/kg, and could be relieved by dexamethasone (DXM). Gradient damage was observed in kidney and liver in rats with gradient MC-RR doses of 574.7, 287.3, and 143.7 μg/kg. No significant water metabolic changes or kidney injuries were observed in mice treated with MC-RR doses of 210.0, 105.0, and 52.5 μg/kg. In the continuous exposure test, in which mice were administrated with 140.0, 70.0, and 35.0 μg/kg MC-RR for 28 days, mice in the 140.0 μg/kg group presented increasing polydipsia, polyuria, and liver damage. However, no anatomic or histological changes, including related serological and urinary indices, were found in the kidney. In summary, abnormal water metabolism can be induced by MC-RR in rats through kidney injury in single dose exposure; the kidney of SD rats is more sensitive to MC-RR than that of KM mouse; and polydipsia and polyuria in mice exposed to MC-RR for 28 days occurred but could not be attributed to kidney damage.
本研究以 SD 大鼠和 KM 小鼠为实验动物，观察了微囊藻毒素 RR（MC-RR）对水代谢的影响。在单次染毒毒性实验中，574.7μg/kg 剂量组大鼠出现多饮、多尿、血尿和蛋白尿，地塞米松（DXM）可缓解上述症状；随染毒剂量增加，大鼠肾脏和肝脏出现梯度损伤。MC-RR 剂量为 210.0、105.0 和 52.5μg/kg 时，KM 小鼠水代谢未见明显变化，也未见肾脏损伤。连续染毒实验中，140.0、70.0 和 35.0μg/kg 剂量组小鼠多饮、多尿，肝脏损伤逐渐加重；但肾脏未见解剖和组织学改变，相关血清学和尿液指标也未见异常。综上，MC-RR 单次染毒可通过肾脏损伤引起大鼠水代谢异常；SD 大鼠对 MC-RR 的敏感性高于 KM 小鼠；MC-RR 连续染毒 28 天引起的多饮、多尿与肾脏损伤无关。
