Pereira-da-Silva Tiago, Napoleão Patrícia, Costa Marina C, Gabriel André F, Selas Mafalda, Silva Filipa, Enguita Francisco J, Ferreira Rui Cruz, Carmo Miguel Mota
Department of Cardiology, Hospital de Santa Marta, Centro Hospitalar Universitário de Lisboa Central, 1169-024 Lisbon, Portugal.
NOVA Medical School, Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, 1169-056 Lisbon, Portugal.
Diagnostics (Basel). 2021 Feb 16;11(2):318. doi: 10.3390/diagnostics11020318.
The mechanisms that regulate the systemic extent of atherosclerosis are not fully understood. We investigated whether the expression of circulating miRNAs is associated with the extent of stable atherosclerosis to a single territory or multiple territories (polyvascular) and with the severity of atherosclerosis in each territory. Ninety-four participants were prospectively recruited and divided into five age- and sex-matched groups: presenting no atherosclerosis, isolated coronary atherosclerosis, coronary and lower extremity atherosclerosis, coronary and carotid atherosclerosis, and atherosclerosis of the coronary, lower extremity, and carotid territories. The expression of six circulating miRNAs with distinct biological roles was assessed. The expression of miR-27b and miR-146 differed across groups ( < 0.05), showing a decrease in the presence of atherosclerosis, particularly in the three territories. miR-27b and miR-146 expression decreased in association with a higher severity of coronary, lower extremity, and carotid atherosclerosis. Polyvascular atherosclerosis involving the three territories was independently associated with a decreased miR-27b and miR-146 expression. Both miRNAs presented an area under the curve of ≥0.75 for predicting polyvascular atherosclerosis involving the three territories. To conclude, miR-27b and miR-146 were associated with the presence of severe polyvascular atherosclerosis and with the atherosclerosis severity in each territory. Both are potential biomarkers of severe systemic atherosclerosis.
调节动脉粥样硬化全身范围的机制尚未完全明确。我们研究了循环miRNA的表达是否与单一区域或多个区域（多血管）的稳定动脉粥样硬化范围以及每个区域的动脉粥样硬化严重程度相关。前瞻性招募了94名参与者，并将其分为五个年龄和性别匹配的组：无动脉粥样硬化、孤立性冠状动脉粥样硬化、冠状动脉和下肢动脉粥样硬化、冠状动脉和颈动脉粥样硬化，以及冠状动脉、下肢和颈动脉区域的动脉粥样硬化。评估了六种具有不同生物学作用的循环miRNA的表达。miR-27b和miR-146的表达在各组间存在差异（<0.05），在存在动脉粥样硬化时表达降低，尤其是在三个区域。miR-27b和miR-146的表达随着冠状动脉、下肢和颈动脉粥样硬化严重程度的增加而降低。涉及三个区域的多血管动脉粥样硬化与miR-27b和miR-146表达降低独立相关。两种miRNA在预测涉及三个区域的多血管动脉粥样硬化时曲线下面积均≥0.75。总之，miR-27b和miR-146与严重多血管动脉粥样硬化的存在以及每个区域的动脉粥样硬化严重程度相关。两者都是严重全身性动脉粥样硬化的潜在生物标志物。
