Pereira-da-Silva Tiago, Coutinho Cruz Madalena, Carrusca Catarina, Cruz Ferreira Rui, Napoleão Patrícia, Mota Carmo Miguel
Department of Cardiology, Hospital de Santa Marta, Centro Hospitalar de Lisboa CentralLisbon, Portugal.
NOVA Medical School, Universidade NOVA de LisboaLisbon, Portugal.
Am J Cardiovasc Dis. 2018 Feb 5;8(1):1-13. eCollection 2018.
Atherosclerosis is associated with altered circulating microRNA profiles. It is yet unclear whether the expression of these potential biomarkers differs according to the location of atherosclerosis. We assessed whether atherosclerosis of different arterial territories, except the coronary, is associated with specific circulating microRNA profiles.
A systematic search in PubMed, Web of Science, Embase, and Cochrane Library was carried out using a retrieval strategy including MESH and non-MSH terms. Eligible studies have compared circulating microRNA profiles between individuals with and without stable atherosclerotic disease of large or medium size arteries. The review protocol was registered in PROSPERO database (reference CRD42017073846).
Eighteen studies were selected for qualitative synthesis: ten focused on carotid, six on lower limbs, and two on renal arteries atherosclerosis, none reporting on other locations. A common microRNA profile to different atherosclerotic disease locations was identified, including deregulation of miR-21, miR-30, miR-126, and miR-221-3p. Specific microRNA profiles for each territory were also identified, with consistency across studies, such as deregulation of miR-21 and miR-29 in carotid atherosclerosis, and let 7e, miR-27b, miR-130a, and miR-210 in lower limbs atherosclerosis. The robustness of the results was very high for let 7e, miR-29, miR-30, considering both the adjustment of microRNA expression for baseline variables and the replication of results in different studies (miR-29 in carotid, let 7e in lower limbs, and miR-30 in carotid and lower limbs atherosclerosis). Globally, the deregulated microRNAs are associated with control of angiogenesis, endothelial cell function, inflammation, cholesterol metabolism, oxidative stress and extracellular matrix composition.
A common microRNA profile to different atherosclerotic disease locations and specific microRNA profiles for each territory were identified. These findings may provide insights into pathophysiology and be useful for selecting potential biomarkers for clinical practice. To the best of our knowledge, no systematic data on this subject has been reported.
动脉粥样硬化与循环中微小RNA谱的改变有关。目前尚不清楚这些潜在生物标志物的表达是否因动脉粥样硬化的位置而异。我们评估了除冠状动脉外不同动脉区域的动脉粥样硬化是否与特定的循环微小RNA谱相关。
在PubMed、科学网、Embase和Cochrane图书馆进行系统检索，检索策略包括医学主题词（MESH）和非MESH词。符合条件的研究比较了有和没有大中型动脉稳定动脉粥样硬化疾病的个体之间的循环微小RNA谱。该综述方案已在PROSPERO数据库中注册（参考文献CRD42017073846）。
选择了18项研究进行定性综合分析：10项聚焦于颈动脉，6项聚焦于下肢，2项聚焦于肾动脉粥样硬化，没有关于其他部位的报道。确定了不同动脉粥样硬化疾病部位的常见微小RNA谱，包括miR-21、miR-30、miR-126和miR-221-3p的失调。还确定了每个区域的特定微小RNA谱，各研究结果一致，如颈动脉粥样硬化中miR-21和miR-29的失调，以及下肢动脉粥样硬化中let-7e、miR-27b、miR-130a和miR-210的失调。考虑到对基线变量的微小RNA表达调整以及不同研究结果的重复性（颈动脉中的miR-29、下肢中的let-7e以及颈动脉和下肢动脉粥样硬化中的miR-30），let-7e、miR-29、miR-30结果的稳健性非常高。总体而言，失调的微小RNA与血管生成、内皮细胞功能、炎症、胆固醇代谢、氧化应激和细胞外基质组成的调控有关。
确定了不同动脉粥样硬化疾病部位的常见微小RNA谱以及每个区域的特定微小RNA谱。这些发现可能有助于深入了解病理生理学，并有助于选择临床实践中的潜在生物标志物。据我们所知，尚未报道关于该主题的系统数据。
