Pešić Maja, Kličan-Jaić Katarina, Vučić Marinko, Kuna Krunoslav, Košec Andro, Čipak Gašparović Ana
Department of Anesthesiology, Intensive Care Medicine and Pain Management, University Hospital Center "Sestre milosrdnice", HR-10000 Zagreb, Croatia.
Department of Gynecology and Obstetrics, University Hospital Center "Sestre Milosrdnice", HR-10000 Zagreb, Croatia.
J Clin Med. 2021 Feb 19;10(4):855. doi: 10.3390/jcm10040855.
Infertility is one of the major medical problems nowadays. Couples who opt for In Vitro Fertilization (IVF) face a great deal of stress which certainly affects the outcome of the procedure. Therefore, we aimed to reduce the stress during the oocyte retrieval procedure by applying midazolam. Total oxidant (TOC) and antioxidant (TAC) capacities of serum, as well as glutathione (GSH) content and catalase activity, were measured in both control and midazolam groups. Follicular fluid was also tested for oxidant capacity and IL1β. Results implied that the midazolam group increased TAC at the end of the procedure. At the same time, the control group decreased GSH at the beginning of the procedure, and both groups decreased catalase activity at the end of the procedure. The results imply that stress during the procedure affects oxidative and antioxidative parameters of the patients, but did not affect the frequency of the pregnancy at the end of this pilot study. Yet, the results imply that oxidative and antioxidative mechanisms during IVF should be investigated in detail as they could affect the outcome of IVF.
不孕症是当今主要的医学问题之一。选择体外受精（IVF）的夫妇面临着巨大的压力，这无疑会影响该 procedure 的结果。因此，我们旨在通过应用咪达唑仑来减轻取卵 procedure 期间的压力。在对照组和咪达唑仑组中均测量了血清的总氧化剂（TOC）和抗氧化剂（TAC）能力，以及谷胱甘肽（GSH）含量和过氧化氢酶活性。还对卵泡液进行了氧化剂能力和 IL1β检测。结果表明，咪达唑仑组在该 procedure 结束时 TAC 增加。同时，对照组在该 procedure 开始时 GSH 降低，且两组在该 procedure 结束时过氧化氢酶活性均降低。结果表明，该 procedure 期间的压力会影响患者的氧化和抗氧化参数，但在这项初步研究结束时并未影响妊娠频率。然而，结果表明，IVF 期间的氧化和抗氧化机制应进行详细研究，因为它们可能会影响 IVF 的结果。
