Pasqualotto Eleonora B, Agarwal Ashok, Sharma Rakesh K, Izzo Vicente M, Pinotti José A, Joshi Narendra J, Rose Bruce I
Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
Fertil Steril. 2004 Apr;81(4):973-6. doi: 10.1016/j.fertnstert.2003.11.021.
OBJECTIVE(S): To measure levels of lipid peroxidation (LPO) and total antioxidant capacity (TAC) in the follicular fluid from patients undergoing IVF and to examine the association among LPO and TAC and oocyte maturity, embryo quality, fertilization, cleavage, and pregnancy rates.
Prospective study.
Tertiary care medical center.
PATIENT(S): Forty-one patients undergoing IVF.
INTERVENTION(S): Clear follicular fluid specimens were collected at oocyte retrieval.
MAIN OUTCOME MEASURE(S): Follicular fluid LPO and TAC levels measured by the thiobarbituric acid and the chemiluminescence method, respectively.
RESULT(S): The mean LPO level was 0.95 micromol malonaldehyde/L, and the mean TAC level was 819.16 Trolox equivalents. No correlation was seen among LPO or TAC levels and oocyte maturity, fertilization, cleavage, and embryo quality. The pregnancy rate was 31.7%. Pregnant patients were significantly younger than the patients who did not become pregnant (30.53 +/- 3.5 years vs. 34.32 +/- 3.98 years). After adjusting for age, a positive correlation was seen between pregnancy rate and LPO and between pregnancy rate and TAC.
CONCLUSION(S): Both LPO and TAC levels were positively correlated with the pregnancy rate. Lipid peroxidation may be a good marker of metabolic activity within the follicle, and some amounts may be necessary to establish a pregnancy.
测定接受体外受精（IVF）患者卵泡液中脂质过氧化（LPO）水平和总抗氧化能力（TAC），并研究LPO、TAC与卵母细胞成熟度、胚胎质量、受精、卵裂及妊娠率之间的关联。
前瞻性研究。
三级医疗中心。
41例接受IVF的患者。
在取卵时收集清亮的卵泡液标本。
分别采用硫代巴比妥酸法和化学发光法测定卵泡液LPO和TAC水平。
LPO平均水平为0.95微摩尔丙二醛/升，TAC平均水平为819.16特洛克斯当量。未发现LPO或TAC水平与卵母细胞成熟度、受精、卵裂及胚胎质量之间存在相关性。妊娠率为31.7%。妊娠患者明显比未妊娠患者年轻（30.53±3.5岁 vs. 34.32±3.98岁）。校正年龄后，妊娠率与LPO以及妊娠率与TAC之间呈正相关。
LPO和TAC水平均与妊娠率呈正相关。脂质过氧化可能是卵泡内代谢活性的良好标志物，建立妊娠可能需要一定量的脂质过氧化。
