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通过仿生矿化制备乳铁蛋白/磷酸钙改性多孔钛：有效预防感染并具有出色的骨诱导性

Lactoferrin/Calcium Phosphate-Modified Porous Ti by Biomimetic Mineralization: Effective Infection Prevention and Excellent Osteoinduction.

作者信息

Chen Song, He Yuanli, Zhong Linna, Xie Wenjia, Xue Yiyuan, Wang Jian

机构信息

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

出版信息

Materials (Basel). 2021 Feb 19;14(4):992. doi: 10.3390/ma14040992.

DOI:10.3390/ma14040992
PMID:33669904
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7923298/
Abstract

The surface modification of titanium (Ti) can enhance the osseointegration and antibacterial properties of implants. In this study, we modified porous Ti discs with calcium phosphate (CaP) and different concentrations of Lactoferrin (LF) by biomimetic mineralization and examined their antibacterial effects and osteogenic bioactivity. Firstly, scanning electron microscopy (SEM), the fluorescent tracing method, X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and the releasing kinetics of LF were utilized to characterize the modified Ti surface. Then, the antibacterial properties against and were investigated. Finally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell differentiation, extracellular matrix mineralization, and cytotoxicity. The results showed that the porous Ti discs were successfully modified with CaP and LF, and that the LF-M group (200 μg/mL LF in simulated body fluid) could mildly release LF under control. Further, the LF-M group could effectively inhibit the adhesion and proliferation of and and enhance the osteogenic differentiation in vitro with a good biocompatibility. Consequently, LF-M-modified Ti may have potential applications in the field of dental implants to promote osseointegration and prevent the occurrence of peri-implantitis.

摘要

钛（Ti）的表面改性可以增强植入物的骨整合和抗菌性能。在本研究中，我们通过仿生矿化用磷酸钙（CaP）和不同浓度的乳铁蛋白（LF）对多孔钛盘进行改性，并研究了它们的抗菌效果和成骨生物活性。首先，利用扫描电子显微镜（SEM）、荧光示踪法、X射线光电子能谱（XPS）、傅里叶变换红外光谱（FTIR）、能量色散X射线光谱（EDX）、X射线衍射（XRD）以及LF的释放动力学来表征改性的钛表面。然后，研究了对[具体细菌名称1]和[具体细菌名称2]的抗菌性能。最后，进行了体外细胞学检查，包括细胞黏附、细胞分化、细胞外基质矿化和细胞毒性的评估。结果表明，多孔钛盘成功地用CaP和LF进行了改性，并且LF-M组（模拟体液中含200μg/mL LF）能够在可控条件下温和释放LF。此外，LF-M组能够有效抑制[具体细菌名称1]和[具体细菌名称2]的黏附和增殖，并在体外增强成骨分化，具有良好的生物相容性。因此，LF-M改性钛在牙种植领域可能具有潜在应用，以促进骨整合并预防种植体周围炎的发生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fb5/7923298/8daad323be4e/materials-14-00992-g009.jpg
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