Fukuda A, Noda Y, Mori T, Mori C, Hashimoto H, Hoshino K
Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Japan.
J In Vitro Fert Embryo Transf. 1988 Feb;5(1):25-30. doi: 10.1007/BF01138866.
Hyperprolactinemia is one of the major causative factors of infertility. However, the effect of prolactin on gametes during in vitro fertilization has not been elucidated. In the present study, the effects of mouse prolactin on the motility of spermatozoa, in vitro fertilization, and in vitro development of the zygote were investigated in mice using media containing three different concentrations (10, 50, and 100 ng/ml) of mouse prolactin. The development of unfertilized and fertilized oocytes (zygotes) was not affected in vitro by prolactin regardless of the amount of prolactin added to culture media. However, the fertilizing capacity of the spermatozoa was suppressed when they were preincubated for 90 min in culture media containing prolactin at concentrations of 50 and 100 ng/ml. The motility of spermatozoa was not affected by prolactin regardless of the concentration of prolactin used for preincubation. The present study indicates that prolactin may have some effects on the capacitation process of spermatozoa in vitro. This result should be taken into consideration in in vitro fertilization and embryo transfer procedures in humans.
高催乳素血症是导致不孕的主要因素之一。然而，催乳素在体外受精过程中对配子的影响尚未阐明。在本研究中，使用含有三种不同浓度（10、50和100 ng/ml）小鼠催乳素的培养基，研究了小鼠催乳素对小鼠精子活力、体外受精以及合子体外发育的影响。无论向培养基中添加多少催乳素，未受精和已受精的卵母细胞（合子）的体外发育均不受催乳素影响。然而，当精子在含有50和100 ng/ml催乳素的培养基中预孵育90分钟时，其受精能力受到抑制。无论用于预孵育的催乳素浓度如何，精子活力均不受催乳素影响。本研究表明，催乳素可能在体外对精子的获能过程有一定影响。这一结果在人类体外受精和胚胎移植程序中应予以考虑。
