Nioi Matteo, Napoli Pietro Emanuele, Demontis Roberto, Locci Emanuela, Fossarello Maurizio, d'Aloja Ernesto
Forensic Medicine Unit, Department of Clinical Sciences and Public Health, University of Cagliari, 09124 Cagliari, Italy.
Eye Clinic, Department of Surgical Science, University of Cagliari, 09124 Cagliari, Italy.
Diagnostics (Basel). 2021 Feb 28;11(3):413. doi: 10.3390/diagnostics11030413.
Postmortem analysis of the ocular globe is an important topic for forensic pathology and transplantology. Although crucial elements may be gathered from examining cadaveric eyes, the latter do not routinely undergo in-depth analysis. The paucity of quantitative and objective data that are obtainable using current, invasive necroscopic techniques is the main reason for the limited interest in this highly specialized procedure. The aim of the current study is to describe and to object for the first time the postmortem ocular changes by mean of portable optical coherence tomography for evaluating ocular tissues postmortem. The design involved the postmortem analysis (in situ, and without enucleation) of 12 eyes by portable spectral-domain Optical Coherence Tomography. The scans were performed, in corneal, retinal and angle modality at different intervals: <6 h, 6th, 12th, and 24th hour and after autopsy (25th-72nd hour). The morphological changes in the cornea, sclera, vitreous humor and aqueous humor were easy to explore and objectify in these tissues in first 72 h postmortem. On the other hand, the "in situ" observation of the retina was difficult due to the opacification of the lenses in the first 24 h after death.
眼球的尸检分析是法医病理学和移植学的一个重要课题。尽管通过检查尸体眼睛可以收集到关键要素，但后者通常不会进行深入分析。使用当前侵入性尸检技术可获得的定量和客观数据匮乏，是人们对这种高度专业化程序兴趣有限的主要原因。本研究的目的是首次通过便携式光学相干断层扫描描述并客观呈现死后眼部变化，以评估死后的眼部组织。该设计包括通过便携式光谱域光学相干断层扫描对12只眼睛进行尸检分析（原位，不摘除眼球）。扫描在角膜、视网膜和房角模式下于不同时间间隔进行：<6小时、6小时、12小时、24小时以及尸检后（25 - 72小时）。在死后的前72小时内，角膜、巩膜、玻璃体和房水的形态变化在这些组织中易于探究和客观呈现。另一方面，由于死后最初24小时内晶状体混浊，视网膜的“原位”观察较为困难。
