Schössler V W, Töpfer G, Rüger H J
Staatliches Institut für Immunpräparate und Nährmedien, Berlin, DDR.
J Clin Chem Clin Biochem. 1988 Feb;26(2):75-8.
The principle is based on the competitive binding of the enzyme-labelled CRP (enzyme: alkaline phosphatase) and the serum CRP to solid phase-bound antibodies. The detection limit was 10 micrograms/l. The precision in the series in the border-line "normal" to "pathological" (5 mg/l) was 2.9%; the precision from day to day at the concentration of 5 mg/l was 4.5%. Only 2 h are required for the whole test.
该原理基于酶标记的CRP(酶:碱性磷酸酶)与血清CRP对固相结合抗体的竞争性结合。检测限为10微克/升。在“正常”至“病理”边界值(5毫克/升)系列中的精密度为2.9%;在5毫克/升浓度下,每日精密度为4.5%。整个检测只需2小时。