He Yingting, Zhou Xiaofeng, Zheng Rongrong, Jiang Yao, Yao Zhixiang, Wang Xilong, Zhang Zhe, Zhang Hao, Li Jiaqi, Yuan Xiaolong
Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Guangdong Dexing Food Co., Ltd., Shantou 515100, China.
Animals (Basel). 2021 Feb 17;11(2):521. doi: 10.3390/ani11020521.
In mammals, the exocyst complex component 4 () gene has often been reported to be involved in vesicle transport. The SNP rs81471943 (C/T) is located in the intron of porcine , while six quantitative trait loci (QTL) within 5-10 Mb around are associated with ovary weight, teat number, total offspring born alive, and corpus luteum number. However, the molecular mechanisms between and the reproductive performance of pigs remains to be elucidated. In this study, rs81471943 was genotyped from a total of 994 Duroc sows, and the genotype and allele frequency of SNP rs81471943 (C/T) were statistically analyzed. Then, the associations between SNP rs81471943 and four reproductive traits, including number of piglets born alive (NBA), litter weight at birth (LWB), number of piglets weaned (NW), and litter weight at weaning (LWW), were determined. Sanger sequencing and PCR restriction fragment length polymorphism (PCR-RFLP) were utilized to identify the rs81471943 genotype. We found that the genotype frequency of CC was significantly higher than that of CT and TT, and CC was the most frequent genotype for NBA, LWB, NW, and LWW. Moreover, 5'-deletion and luciferase assays identified a positive transcription regulatory element in the promoter. After exploring the promoter, SNP -1781G/A linked with SNP rs81471943 (C/T) were identified by analysis of the transcription activity of the haplotypes, and SNP -1781 G/A may influence the potential binding of , E26 transformation specific sequence -like 1 transcription factor (), and myeloid zinc finger 1 (). These findings provide useful information for identifying a molecular marker of -assisted selection in pig breeding.
在哺乳动物中，外泌体复合体成分4（）基因常被报道参与囊泡运输。单核苷酸多态性（SNP）rs81471943（C/T）位于猪的内含子中，而在其周围5 - 10 Mb范围内的6个数量性状基因座（QTL）与卵巢重量、乳头数、产活仔总数和黄体数相关。然而，与猪繁殖性能之间的分子机制仍有待阐明。在本研究中，对总共994头杜洛克母猪的rs81471943进行基因分型，并对SNP rs81471943（C/T）的基因型和等位基因频率进行统计分析。然后，确定SNP rs81471943与四个繁殖性状之间的关联，包括产活仔数（NBA）、初生窝重（LWB）、断奶仔猪数（NW）和断奶窝重（LWW）。利用桑格测序和聚合酶链反应 - 限制性片段长度多态性（PCR - RFLP）来鉴定rs81471943基因型。我们发现CC基因型频率显著高于CT和TT基因型频率，并且CC是NBA、LWB、NW和LWW最常见的基因型。此外，5' - 缺失和荧光素酶分析在启动子中鉴定出一个正向转录调控元件。在探索启动子后，通过单倍型转录活性分析鉴定出与SNP rs81471943（C/T）连锁的SNP - 1781G/A，并且SNP - 1781G/A可能影响、E26转化特异性序列样1转录因子（）和髓样锌指1（）的潜在结合。这些发现为鉴定猪育种中辅助选择的分子标记提供了有用信息。
