Gan QianFu, Li YiRan, Liu QingHua, Lund M, Su GuoSheng, Liang XueWu
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Department of Molecular Biology and Genetics, Center for Quantitative Genetics and Genomics, Aarhus University, 8000, Aarhus, Denmark.
Trop Anim Health Prod. 2020 Jul;52(4):1655-1660. doi: 10.1007/s11250-019-02170-z. Epub 2019 Dec 18.
To further understand the genetic structure that is associated with insulin (INS) and thyroid hormones (TH), including triiodothyronine (T) and thyroxine (T), in Chinese Holstein cows, we conducted a genome-wide association study (GWAS) of thyroid hormones and insulin in cows. We conducted GWAS analysis on 1217 Chinese Holstein cows raised in southern China and found 19 significant single nucleotide polymorphisms (SNPs) in this study: 10 SNPs were associated with INS, 5 SNPs were associated with T, and 4 SNPs were associated with T. In our study, the GWAS method was used for preliminary screening on related genes of traits, and due to insufficient relevant literature, a functional analysis of genes could only be based on human studies. We observed that DGKB from Bos taurus chromosome (BTA)4 is strongly associated with insulin secretion. We found that EXOC4 gene was significantly correlated with T and T traits. Another significant SNP was located in the CYP7A1 gene, which has been confirmed to be affected by thyroid hormones.
为了进一步了解中国荷斯坦奶牛中与胰岛素（INS）和甲状腺激素（TH）相关的遗传结构，包括三碘甲状腺原氨酸（T3）和甲状腺素（T4），我们对奶牛的甲状腺激素和胰岛素进行了全基因组关联研究（GWAS）。我们对在中国南方饲养的1217头中国荷斯坦奶牛进行了GWAS分析，在本研究中发现了19个显著的单核苷酸多态性（SNP）：10个SNP与INS相关，5个SNP与T3相关，4个SNP与T4相关。在我们的研究中，GWAS方法用于对性状相关基因进行初步筛选，由于相关文献不足，基因功能分析只能基于人类研究。我们观察到来自牛染色体（BTA）4的DGKB与胰岛素分泌密切相关。我们发现EXOC4基因与T3和T4性状显著相关。另一个显著的SNP位于CYP7A1基因中，该基因已被证实受甲状腺激素影响。
