Bioanalytical and Pharmacokinetic Study Group, Korea Institute of Toxicology, Daejeon 34114, Korea.
Animal Model Research Group, Jeonbuk Department of Inhalation Research, Korea Institute of Toxicology, Jeongeup, Jeollabuk-do, Daejeon 53212, Korea.
Molecules. 2021 Feb 22;26(4):1177. doi: 10.3390/molecules26041177.
Sialyllactose (SL), an acidic oligosaccharide, has immune-protective effects against pathogens and helps with the development of the immune system and intestinal microorganisms. To elucidate the pharmacokinetic characterization after oral administration to rats, the simultaneous quantification method for 3'-SL and 6'-SL in rat plasma was validated, using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in an electrospray ionization (ESI) mode. Several types of columns [C18, amide, and hydrophilic interaction liquid chromatography (HILIC) phase] were used to separate the peaks of 3'-SL and 6'-SL, which improved chromatographic selectivity. Ultimately, the HILIC phase column had a good peak shape and quick resolution, with a mobile phase comprising ammonium acetate buffer and acetonitrile obtained by gradient elution. In addition, the simultaneous quantification of 3'-SL and 6'-SL in rat plasma samples were adequately applied to pharmacokinetic study.
唾液乳糖（SL）是一种酸性低聚糖，具有抗病原体的免疫保护作用，有助于免疫系统和肠道微生物的发育。为了阐明口服给予大鼠后的药代动力学特征，使用电喷雾电离（ESI）模式的液相色谱-串联质谱（LC-MS/MS）对大鼠血浆中 3'-SL 和 6'-SL 的同时定量方法进行了验证。使用了几种类型的色谱柱[C18、酰胺和亲水作用色谱（HILIC）相]来分离 3'-SL 和 6'-SL 的峰，从而提高了色谱选择性。最终，HILIC 相色谱柱具有良好的峰形和快速的分辨率，通过梯度洗脱得到包含乙酸铵缓冲液和乙腈的流动相。此外，大鼠血浆样品中 3'-SL 和 6'-SL 的同时定量充分应用于药代动力学研究。
