State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Int J Environ Res Public Health. 2021 Feb 23;18(4):2168. doi: 10.3390/ijerph18042168.
Human disturbance and vegetation are known to affect soil microorganisms. However, the interacting effects of pavement and plant species on soil bacterial communities have received far less attention. In this study, we collected soil samples from pine (), ash (), and maple () stands that grew in impervious, pervious, and no pavement blocks to investigate the way pavement, tree species, and their interaction influence soil bacterial communities by modifying soil physicochemical properties. Soil bacterial community composition and diversity were evaluated by bacterial 16S amplicon sequencing. The results demonstrated that soil bacterial community composition and diversity did differ significantly across pavements, but not with tree species. The difference in soil bacterial community composition across pavements was greater in pine stands than ash and maple stands. Soil bacterial diversity and richness indices decreased beneath impervious pavement in pine stands, and only bacterial richness indices decreased markedly in ash stands, but neither showed a significant difference across pavements in maple stands. In addition, bacterial diversity did not differ dramatically between pervious pavement and no pavement soil. Taken together, these results suggest that pavement overwhelmed the effects of tree species on soil bacterial communities, and had a greater effect on soil bacterial communities in pine stands, followed by ash and maple stands. This study highlights the importance of anthropogenic disturbance, such as pavement, which affects soil microbial communities.
人类干扰和植被被认为会影响土壤微生物。然而，铺砌路面和植物物种对土壤细菌群落的相互影响却很少受到关注。本研究从不透水、透水和无铺砌路面的油松（）、白蜡（）和枫香（）林分中采集土壤样本，通过改变土壤理化性质，研究铺砌路面、树种及其相互作用对土壤细菌群落的影响方式。通过细菌 16S 扩增子测序评估土壤细菌群落组成和多样性。结果表明，土壤细菌群落组成和多样性在不同铺砌路面之间存在显著差异，但与树种无关。在油松林分中，不同铺砌路面之间土壤细菌群落组成的差异大于白蜡和枫香林分。不透水路面下油松林分的土壤细菌多样性和丰富度指数降低，而只有白蜡林分的细菌丰富度指数显著降低，但枫香林分在各铺砌路面之间均无显著差异。此外，透水路面和无铺砌路面的土壤细菌多样性差异不显著。总的来说，这些结果表明，铺砌路面对土壤细菌群落的影响超过了树种的影响，对油松林分的土壤细菌群落影响更大，其次是白蜡和枫香林分。本研究强调了人为干扰（如铺砌路面）对土壤微生物群落的重要性。
