Moawad Adel R, Salama Ali, Badr Magdy R, Fathi Mohamed
Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Department of AI and ET, Animal Reproduction Research Institute, Agriculture Research Centre, Giza 12556, Egypt.
Animals (Basel). 2021 Feb 23;11(2):581. doi: 10.3390/ani11020581.
This study aimed to investigate the effect of L-Carnitine (LC) supplementation during in vitro maturation (IVM) of canine oocytes on nuclear maturation, fertilization status, and preimplantation development. Cumulus-oocyte complexes (COCs) collected from the ovaries of ovariohysterectomized female dogs were matured in vitro for 72 h in a TCM-199 medium supplemented with (0.1, 0.3, 0.6, 1.0, or 2.0 mg/mL) or without (0.0 mg/mL) LC. Matured oocytes were fertilized in vitro with frozen-thawed spermatozoa, and zygotes were cultured in a SOF medium for 7 days. IVM rates were higher ( 0.05) in 0.3 and 0.6 mg/mL LC supplemented groups than in the control (0.0 mg/mL LC) and other LC groups. Fertilization (18 h postinsemination (pi)) and cleavage (2-16-cell stage at day 3 pi) rates were higher ( 0.05) in the 0.6 mg/mL LC group than in the control and 0.1, 1.0, and 2 mg/mL LC supplemented groups. Interestingly, 4.5% of fertilized oocytes developed to morula (day 5 pi) in the 0.6 mg/mL LC group, which was higher ( 0.05) than those developed in the 0.3 mg/mL group (1.0%). No cleaved embryos developed to morula in other groups. In conclusion, LC supplementation at 0.6 mg/mL during IVM of canine oocytes improved their maturation, fertilization, and preimplantation embryo development rates following IVF and in vitro culture (IVC).
本研究旨在探讨在犬卵母细胞体外成熟（IVM）过程中补充左旋肉碱（LC）对核成熟、受精状态和植入前发育的影响。从卵巢子宫切除的雌性犬卵巢中收集的卵丘-卵母细胞复合体（COCs）在添加（0.1、0.3、0.6、1.0或2.0 mg/mL）或不添加（0.0 mg/mL）LC的TCM-199培养基中体外成熟72小时。成熟的卵母细胞用冻融精子进行体外受精，受精卵在SOF培养基中培养7天。添加0.3和0.6 mg/mL LC的组的IVM率高于对照组（0.0 mg/mL LC）和其他LC组（P<0.05）。0.6 mg/mL LC组的受精率（授精后18小时（pi））和卵裂率（授精后第3天的2-16细胞阶段）高于对照组以及添加0.1、1.0和2 mg/mL LC的组（P<0.05）。有趣的是，0.6 mg/mL LC组中4.5%的受精卵发育至桑葚胚（授精后第5天），高于0.3 mg/mL组（1.0%）（P<0.05）。其他组中没有卵裂胚胎发育至桑葚胚。总之，犬卵母细胞IVM期间补充0.6 mg/mL的LC可提高其在体外受精（IVF）和体外培养（IVC）后的成熟、受精和植入前胚胎发育率。
